152820

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 88900..89543 Replicon plasmid p52148_NDM_5
Accession NZ_CP050384
Organism Escherichia coli strain 52148

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag HB638_RS23840 Protein ID WP_001034044.1
Coordinates 89127..89543 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag HB638_RS23835 Protein ID WP_001261286.1
Coordinates 88900..89130 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB638_RS23815 85531..86286 - 756 WP_000852146.1 replication initiation protein RepE -
HB638_RS23820 87008..87814 - 807 WP_000016970.1 site-specific integrase -
HB638_RS23825 87815..88120 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
HB638_RS23830 88122..88340 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
HB638_RS23835 88900..89130 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HB638_RS23840 89127..89543 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
HB638_RS23845 89618..91183 + 1566 WP_001128474.1 AAA family ATPase -
HB638_RS23850 91168..92190 + 1023 WP_000361402.1 helicase UvrD -
HB638_RS23855 92444..93130 - 687 Protein_105 IS1 family transposase -
HB638_RS23860 93280..93977 - 698 Protein_106 IS1 family transposase -
HB638_RS24115 94177..94290 + 114 Protein_107 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / mph(A) / blaNDM-5 / sul1 / qacE / aadA2 - 1..121872 121872
- inside Integron qacE / dfrA12 / aadA2 - 2..121837 121835


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T152820 WP_001034044.1 NZ_CP050384:89127-89543 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T152820 NZ_CP066534:720759-721043 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT152820 WP_001261286.1 NZ_CP050384:88900-89130 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT152820 NZ_CP066534:720527-720769 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References