Detailed information of TA system TA152813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4617435..4618011
• Replicon: chromosome
• Accession: NZ_CP050382
• Organism: Escherichia coli strain 52148

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: HB638_RS22095
• Protein ID: WP_001297643.1
• Coordinates: 4617724..4618011 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A080I7H7
• Locus tag: HB638_RS22090
• Protein ID: WP_000063160.1
• Coordinates: 4617435..4617737 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB638_RS22075	4614031..4616181	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
HB638_RS22080	4616231..4616434	+	204	WP_000467859.1	YbdD/YjiX family protein	-
HB638_RS22085	4616445..4617401	+	957	WP_001297640.1	GTPase	-
HB638_RS22090	4617435..4617737	-	303	WP_000063160.1	BrnA antitoxin family protein	Antitoxin
HB638_RS22095	4617724..4618011	-	288	WP_001297643.1	BrnT family toxin	Toxin
HB638_RS22100	4618210..4618906	-	697	Protein_4320	IS1 family transposase	-
HB638_RS22105	4619221..4620453	+	1233	WP_001137030.1	multidrug efflux MFS transporter MdtM	-
HB638_RS22110	4620494..4621774	+	1281	WP_001037390.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4618210..4618419	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T152813 WP_001297643.1 NZ_CP050382:c4618011-4617724 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T152813 NZ_CP066529:105002-105151 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11552.01 Da
• Isoelectric Point: 9.4646

>AT152813 WP_000063160.1 NZ_CP050382:c4617737-4617435 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT152813 NZ_CP066529:c104957-104898 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829GEV9

Antitoxin

• AlphaFold DB: A0A080I7H7