Detailed information of TA system TA152810

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4175210..4175904
• Replicon: chromosome
• Accession: NZ_CP050382
• Organism: Escherichia coli strain 52148

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: HB638_RS19960
• Protein ID: WP_001094398.1
• Coordinates: 4175210..4175578 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HB638_RS19965
• Protein ID: WP_077249034.1
• Coordinates: 4175599..4175904 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB638_RS19940	4170383..4171339	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
HB638_RS19945	4171336..4173534	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
HB638_RS19950	4173544..4174500	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
HB638_RS19955	4174551..4174889	+	339	Protein_3899	LysR family transcriptional regulator	-
HB638_RS19960	4175210..4175578	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
HB638_RS19965	4175599..4175904	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HB638_RS19970	4176008..4176652	-	645	WP_000086755.1	hypothetical protein	-
HB638_RS19975	4176671..4176892	-	222	WP_000692345.1	DUF987 domain-containing protein	-
HB638_RS19980	4176961..4177437	-	477	WP_001424026.1	RadC family protein	-
HB638_RS19985	4177453..4177926	-	474	WP_000855059.1	antirestriction protein	-
HB638_RS19990	4178268..4179089	-	822	WP_001234565.1	DUF945 domain-containing protein	-
HB638_RS19995	4179190..4179398	-	209	Protein_3907	DUF905 family protein	-
HB638_RS20000	4179499..4179954	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	4148720..4189553	40833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T152810 WP_001094398.1 NZ_CP050382:c4175578-4175210 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T152810 NZ_CP066529:c6217-5912 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT152810 WP_077249034.1 NZ_CP050382:c4175904-4175599 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT152810 NZ_CP066529:c6437-6219 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4