Detailed information of TA system TA152798

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2407974..2408809
• Replicon: chromosome
• Accession: NZ_CP050382
• Organism: Escherichia coli strain 52148

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7I6H0K3
• Locus tag: HB638_RS11400
• Protein ID: WP_000854760.1
• Coordinates: 2407974..2408351 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: HB638_RS11405
• Protein ID: WP_001295723.1
• Coordinates: 2408441..2408809 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB638_RS11360	2403494..2403967	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
HB638_RS11365	2404165..2405223	+	1059	WP_001200895.1	FUSC family protein	-
HB638_RS11370	2405395..2405724	+	330	WP_000450409.1	DUF496 family protein	-
HB638_RS11375	2405825..2406007	-	183	WP_024174802.1	ethanolamine utilization protein	-
HB638_RS24055	2406461..2406832	-	372	WP_001295631.1	IS110 family transposase	-
HB638_RS11390	2407648..2407761	-	114	WP_032140621.1	DUF957 domain-containing protein	-
HB638_RS11395	2407774..2407977	-	204	WP_000761705.1	hypothetical protein	-
HB638_RS11400	2407974..2408351	-	378	WP_000854760.1	TA system toxin CbtA family protein	Toxin
HB638_RS11405	2408441..2408809	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HB638_RS11410	2408972..2409193	-	222	WP_000692345.1	DUF987 domain-containing protein	-
HB638_RS11415	2409262..2409738	-	477	WP_001354275.1	RadC family protein	-
HB638_RS11420	2409754..2410227	-	474	WP_001387789.1	antirestriction protein	-
HB638_RS11425	2410569..2411387	-	819	WP_047149353.1	DUF945 domain-containing protein	-
HB638_RS11430	2411506..2412203	-	698	Protein_2223	IS1 family transposase	-
HB638_RS11435	2412259..2412891	+	633	Protein_2224	IS30-like element IS30 family transposase	-
HB638_RS11440	2412901..2413149	-	249	Protein_2225	transposase	-
HB638_RS11445	2413580..2413744	+	165	WP_001128468.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14165.19 Da
• Isoelectric Point: 7.3249

>T152798 WP_000854760.1 NZ_CP050382:c2408351-2407974 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T152798 NZ_CP066527:3337-3603 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT152798 WP_001295723.1 NZ_CP050382:c2408809-2408441 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT152798 NZ_CP066527:3129-3353 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A7I6H0K3

Antitoxin

• AlphaFold DB: S1NM52