Detailed information of TA system TA152789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 8701..9248
• Replicon: plasmid p51015_OXA_244
• Accession: NZ_CP050381
• Organism: Klebsiella pneumoniae strain 51015

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: HB813_RS29340
• Protein ID: WP_000969991.1
• Coordinates: 8967..9248 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6G9U792
• Locus tag: HB813_RS29335
• Protein ID: WP_000079935.1
• Coordinates: 8701..8970 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB813_RS29310	4164..4730	+	567	WP_000469207.1	DUF2726 domain-containing protein	-
HB813_RS29315	4727..5458	+	732	WP_001071488.1	type-F conjugative transfer system pilin acetylase TraX	-
HB813_RS29320	6372..6629	+	258	WP_000083823.1	replication regulatory protein RepA	-
HB813_RS29325	6864..6938	+	75	WP_057113837.1	RepA leader peptide Tap	-
HB813_RS29330	6931..7788	+	858	WP_000130987.1	incFII family plasmid replication initiator RepA	-
HB813_RS29335	8701..8970	+	270	WP_000079935.1	hypothetical protein	Antitoxin
HB813_RS29340	8967..9248	+	282	WP_000969991.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB813_RS29345	9294..10142	+	849	WP_115198154.1	3'-5' exonuclease	-
HB813_RS29350	10259..10741	+	483	WP_001311056.1	hypothetical protein	-
HB813_RS29355	11101..12217	-	1117	Protein_10	IS91 family transposase	-
HB813_RS29360	12217..12582	-	366	WP_000124093.1	hypothetical protein	-
HB813_RS29365	13110..13619	+	510	WP_000115002.1	peptide deformylase	-
HB813_RS29370	13634..13735	+	102	Protein_13	methionyl-tRNA formyltransferase	-
HB813_RS29375	13995..14123	+	129	Protein_14	DUF2164 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-244	-	1..71402	71402
	-	flank	IS/Tn	-	-	11132..11854	722

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10860.56 Da
• Isoelectric Point: 8.4615

>T152789 WP_000969991.1 NZ_CP050381:8967-9248 [Klebsiella pneumoniae]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASSLAGLPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T152789 NZ_CP066523:c5061697-5060780 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10198.53 Da
• Isoelectric Point: 8.8735

>AT152789 WP_000079935.1 NZ_CP050381:8701-8970 [Klebsiella pneumoniae]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLTHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT152789 NZ_CP066523:c5062461-5061703 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A6G9U792