Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 337464..338374 Replicon plasmid p51015_NDM_1
Accession NZ_CP050380
Organism Klebsiella pneumoniae strain 51015

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag HB813_RS29240 Protein ID WP_004026354.1
Coordinates 337904..338374 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag HB813_RS29235 Protein ID WP_004181895.1
Coordinates 337464..337907 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB813_RS29220 334097..335584 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
HB813_RS29225 335670..336092 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
HB813_RS29230 336092..337363 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
HB813_RS29235 337464..337907 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
HB813_RS29240 337904..338374 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
HB813_RS29245 338484..338744 - 261 WP_004026352.1 hypothetical protein -
HB813_RS29250 339466..339624 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
HB813_RS29805 340227..341201 + 975 WP_058842863.1 hypothetical protein -
HB813_RS29260 341399..341776 - 378 WP_029884711.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / armA / msr(E) / mph(E) / qnrS1 / aph(3')-VI / blaNDM-1 / dfrA5 / qacE / sul1 / mph(A) / aph(3')-Ia rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA 1..353810 353810


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T152788 WP_004026354.1 NZ_CP050380:337904-338374 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T152788 NZ_CP066523:c4904880-4904596 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT152788 WP_004181895.1 NZ_CP050380:337464-337907 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT152788 NZ_CP066523:c4905112-4904870 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References