Detailed information of TA system TA152787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 259481..260151
• Replicon: plasmid p51015_NDM_1
• Accession: NZ_CP050380
• Organism: Klebsiella pneumoniae strain 51015

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HB813_RS28765
• Protein ID: WP_004213072.1
• Coordinates: 259708..260151 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HB813_RS28760
• Protein ID: WP_004213073.1
• Coordinates: 259481..259711 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB813_RS28735	255704..256603	-	900	WP_004225022.1	nucleotide-binding protein	-
HB813_RS28740	256593..256883	-	291	WP_004213078.1	hypothetical protein	-
HB813_RS28745	257235..257441	+	207	WP_004213077.1	hypothetical protein	-
HB813_RS28750	257431..257724	-	294	WP_004213076.1	hypothetical protein	-
HB813_RS28755	257740..258873	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HB813_RS28760	259481..259711	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HB813_RS28765	259708..260151	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HB813_RS28770	260165..260581	-	417	Protein_265	restriction endonuclease	-
HB813_RS28775	260585..260878	-	294	Protein_266	transposase	-
HB813_RS29795	261301..261940	+	640	Protein_267	response regulator	-
HB813_RS28785	262456..262862	-	407	Protein_268	GAF domain-containing protein	-
HB813_RS28790	262953..263873	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
HB813_RS28795	263922..264413	-	492	WP_011251327.1	DM13 domain-containing protein	-
HB813_RS28800	264476..264751	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / armA / msr(E) / mph(E) / qnrS1 / aph(3')-VI / blaNDM-1 / dfrA5 / qacE / sul1 / mph(A) / aph(3')-Ia	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA	1..353810	353810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T152787 WP_004213072.1 NZ_CP050380:259708-260151 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T152787 NZ_CP066523:c4802548-4802015 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT152787 WP_004213073.1 NZ_CP050380:259481-259711 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT152787 NZ_CP066523:c4802825-4802559 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620