Detailed information of TA system TA152785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 157373..158118
• Replicon: plasmid p51015_CTX_M_15
• Accession: NZ_CP050379
• Organism: Klebsiella pneumoniae strain 51015

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: HB813_RS27015
• Protein ID: WP_032408901.1
• Coordinates: 157373..157864 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: HB813_RS27020
• Protein ID: WP_014386183.1
• Coordinates: 157852..158118 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB813_RS26990	153057..153812	+	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-
HB813_RS26995	154074..154514	+	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
HB813_RS27000	154511..154861	+	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
HB813_RS27005	154892..156484	+	1593	Protein_169	IS66 family transposase	-
HB813_RS27010	156681..156932	+	252	WP_032408900.1	aldo/keto reductase	-
HB813_RS27015	157373..157864	-	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
HB813_RS27020	157852..158118	-	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
HB813_RS27025	158315..158473	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
HB813_RS27030	158717..159202	-	486	WP_014386185.1	hypothetical protein	-
HB813_RS27035	159392..159664	+	273	WP_032408902.1	hypothetical protein	-
HB813_RS27040	159661..160011	+	351	WP_014386186.1	hypothetical protein	-
HB813_RS27055	160648..160974	+	327	WP_014386187.1	hypothetical protein	-
HB813_RS27060	161036..161248	+	213	WP_014386188.1	hypothetical protein	-
HB813_RS27065	161259..161483	+	225	WP_014386189.1	hypothetical protein	-
HB813_RS27070	161564..161884	+	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HB813_RS27075	161874..162152	+	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
HB813_RS27080	162153..162566	+	414	WP_013023817.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / aph(3')-Ia	-	1..225540	225540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T152785 WP_032408901.1 NZ_CP050379:c157864-157373 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T152785 NZ_CP066523:c2755696-2754374 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT152785 WP_014386183.1 NZ_CP050379:c158118-157852 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT152785 NZ_CP066523:c2755962-2755696 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6