Detailed information of TA system TA152784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 133579..134222
• Replicon: plasmid p51015_CTX_M_15
• Accession: NZ_CP050379
• Organism: Klebsiella pneumoniae strain 51015

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HB813_RS26885
• Protein ID: WP_014386165.1
• Coordinates: 133579..133995 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HB813_RS26890
• Protein ID: WP_032408893.1
• Coordinates: 133992..134222 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB813_RS26860	130199..130630	+	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-
HB813_RS26865	130627..131355	+	729	WP_014386162.1	plasmid SOS inhibition protein A	-
HB813_RS26870	131352..131678	+	327	WP_004152639.1	hypothetical protein	-
HB813_RS26875	131734..132108	+	375	WP_032408891.1	hypothetical protein	-
HB813_RS26880	132288..133256	-	969	WP_074422984.1	IS5 family transposase	-
HB813_RS26885	133579..133995	-	417	WP_014386165.1	PIN domain-containing protein	Toxin
HB813_RS26890	133992..134222	-	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HB813_RS26895	134542..134976	+	435	WP_000103648.1	RidA family protein	-
HB813_RS26900	134981..135655	+	675	WP_014386166.1	DUF1028 domain-containing protein	-
HB813_RS26905	135671..136828	+	1158	WP_032408895.1	acetylornithine deacetylase	-
HB813_RS26910	136999..138147	+	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
HB813_RS26915	138154..139149	+	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / aph(3')-Ia	-	1..225540	225540
	-	flank	IS/Tn	-	-	132288..133256	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T152784 WP_014386165.1 NZ_CP050379:c133995-133579 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T152784 NZ_CP066523:2020700-2020802 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT152784 WP_032408893.1 NZ_CP050379:c134222-133992 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT152784 NZ_CP066523:c2020809-2020664 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT