Detailed information of TA system TA152783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 58960..59696
• Replicon: plasmid p51015_CTX_M_15
• Accession: NZ_CP050379
• Organism: Klebsiella pneumoniae strain 51015

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: HB813_RS26475
• Protein ID: WP_003026803.1
• Coordinates: 58960..59442 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HB813_RS26480
• Protein ID: WP_003026799.1
• Coordinates: 59430..59696 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB813_RS26460	54597..55559	+	963	WP_004152113.1	M48 family metalloprotease	-
HB813_RS26465	55647..56901	+	1255	Protein_65	IS3 family transposase	-
HB813_RS26470	57402..58748	-	1347	WP_020314316.1	ISNCY family transposase	-
HB813_RS26475	58960..59442	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
HB813_RS26480	59430..59696	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HB813_RS26485	59872..60126	-	255	WP_004152108.1	hypothetical protein	-
HB813_RS26490	60202..60459	-	258	WP_004152107.1	hypothetical protein	-
HB813_RS26495	60508..60711	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
HB813_RS26500	60745..61113	-	369	WP_004152105.1	hypothetical protein	-
HB813_RS26505	61157..61651	-	495	WP_004152104.1	DNA-binding protein	-
HB813_RS26510	61682..62257	-	576	WP_004152103.1	hypothetical protein	-
HB813_RS26515	62245..62514	-	270	WP_004152102.1	hypothetical protein	-
HB813_RS26520	62872..63222	+	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
HB813_RS26525	63272..63634	+	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / aph(3')-Ia	-	1..225540	225540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T152783 WP_003026803.1 NZ_CP050379:c59442-58960 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T152783 NZ_CP066523:877987-878394 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT152783 WP_003026799.1 NZ_CP050379:c59696-59430 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT152783 NZ_CP066523:877740-878006 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66