Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 202571..203196 Replicon chromosome
Accession NZ_CP050376
Organism Klebsiella pneumoniae strain 51015

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HB813_RS01005 Protein ID WP_002882817.1
Coordinates 202813..203196 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HB813_RS01000 Protein ID WP_004150355.1
Coordinates 202571..202813 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB813_RS00975 198562..199161 + 600 WP_004151865.1 glucose-1-phosphatase -
HB813_RS00980 199155..200015 + 861 WP_004146232.1 virulence factor BrkB family protein -
HB813_RS00985 200012..200449 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HB813_RS00990 200494..201435 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HB813_RS00995 201449..202366 - 918 WP_004181612.1 alpha/beta hydrolase -
HB813_RS01000 202571..202813 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HB813_RS01005 202813..203196 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HB813_RS01010 203370..204299 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HB813_RS01015 204296..204931 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HB813_RS01020 204928..205830 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T152764 WP_002882817.1 NZ_CP050376:202813-203196 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T152764 NZ_CP066517:350198-350545 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT152764 WP_004150355.1 NZ_CP050376:202571-202813 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT152764 NZ_CP066517:350545-350844 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References