Detailed information of TA system TA152763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 177483..178228
• Replicon: plasmid p50595_NDM_1
• Accession: NZ_CP050374
• Organism: Klebsiella pneumoniae strain 50595

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: HB637_RS29310
• Protein ID: WP_032408901.1
• Coordinates: 177737..178228 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: HB637_RS29305
• Protein ID: WP_014386183.1
• Coordinates: 177483..177749 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB637_RS29250	172965..173669	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HB637_RS29255	173723..174037	-	315	Protein_211	type II toxin-antitoxin system RelE/ParE family toxin	-
HB637_RS29260	174118..174342	-	225	WP_014386189.1	hypothetical protein	-
HB637_RS29265	174353..174565	-	213	WP_014386188.1	hypothetical protein	-
HB637_RS29270	174627..174953	-	327	WP_014386187.1	hypothetical protein	-
HB637_RS29285	175590..175940	-	351	WP_014386186.1	hypothetical protein	-
HB637_RS29290	175937..176209	-	273	WP_032408902.1	hypothetical protein	-
HB637_RS29295	176399..176884	+	486	WP_014386185.1	hypothetical protein	-
HB637_RS29300	177128..177286	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
HB637_RS29305	177483..177749	+	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
HB637_RS29310	177737..178228	+	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
HB637_RS29315	178669..178920	-	252	WP_032408900.1	aldo/keto reductase	-
HB637_RS29320	179117..180709	-	1593	Protein_222	IS66 family transposase	-
HB637_RS29325	180740..181090	-	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
HB637_RS29330	181087..181527	-	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
HB637_RS29335	181789..182544	-	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-15 / aac(3)-IIa / rmtF / ARR-3 / blaNDM-1	-	1..193462	193462

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T152763 WP_032408901.1 NZ_CP050374:177737-178228 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T152763 NZ_CP066517:324227-324595 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT152763 WP_014386183.1 NZ_CP050374:177483-177749 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT152763 NZ_CP066517:324009-324230 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A331KSM6