Detailed information of TA system TA152762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 156350..156993
• Replicon: plasmid p50595_NDM_1
• Accession: NZ_CP050374
• Organism: Klebsiella pneumoniae strain 50595

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HB637_RS29150
• Protein ID: WP_014386165.1
• Coordinates: 156350..156766 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HB637_RS29155
• Protein ID: WP_032408893.1
• Coordinates: 156763..156993 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB637_RS29125	152970..153401	+	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-
HB637_RS29130	153398..154126	+	729	WP_014386162.1	plasmid SOS inhibition protein A	-
HB637_RS29135	154123..154449	+	327	WP_004152639.1	hypothetical protein	-
HB637_RS29140	154505..154879	+	375	WP_032408891.1	hypothetical protein	-
HB637_RS29145	155059..156027	-	969	WP_074422984.1	IS5 family transposase	-
HB637_RS29150	156350..156766	-	417	WP_014386165.1	PIN domain-containing protein	Toxin
HB637_RS29155	156763..156993	-	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HB637_RS29160	157313..157747	+	435	WP_000103648.1	RidA family protein	-
HB637_RS29165	157752..158426	+	675	WP_014386166.1	DUF1028 domain-containing protein	-
HB637_RS29170	158442..159191	+	750	Protein_194	M20/M25/M40 family metallo-hydrolase	-
HB637_RS29175	159209..159906	-	698	Protein_195	IS1 family transposase	-
HB637_RS29180	159960..160376	+	417	Protein_196	M20/M25/M40 family metallo-hydrolase	-
HB637_RS29185	160547..161695	+	1149	WP_014386168.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-15 / aac(3)-IIa / rmtF / ARR-3 / blaNDM-1	-	1..193462	193462
	-	inside	IScluster/Tn	-	-	155059..159586	4527

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T152762 WP_014386165.1 NZ_CP050374:c156766-156350 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T152762 NZ_CP066501:c1280144-1279398 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAAAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT152762 WP_032408893.1 NZ_CP050374:c156993-156763 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT152762 NZ_CP066501:c1279397-1279227 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA