152761

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 118178..118821 Replicon plasmid p50595_NDM_1
Accession NZ_CP050374
Organism Klebsiella pneumoniae strain 50595

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HB637_RS28900 Protein ID WP_001044770.1
Coordinates 118178..118594 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HB637_RS28905 Protein ID WP_001261282.1
Coordinates 118591..118821 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB637_RS28880 115096..115428 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
HB637_RS28885 115475..116350 - 876 WP_000239590.1 class A extended-spectrum beta-lactamase CTX-M-15 -
HB637_RS28890 116663..117360 - 698 Protein_144 IS1 family transposase -
HB637_RS28895 117394..118104 - 711 WP_004152334.1 ATP-binding protein -
HB637_RS28900 118178..118594 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HB637_RS28905 118591..118821 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HB637_RS28910 118778..119237 + 460 Protein_148 hypothetical protein -
HB637_RS28920 119724..120032 + 309 WP_004152336.1 hypothetical protein -
HB637_RS28925 120060..120389 + 330 WP_004152337.1 hypothetical protein -
HB637_RS28930 120415..120813 + 399 WP_004171440.1 hypothetical protein -
HB637_RS28935 120820..121152 + 333 WP_004152339.1 hypothetical protein -
HB637_RS28940 121152..121934 + 783 WP_004152340.1 site-specific integrase -
HB637_RS28945 123148..123621 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / aac(3)-IIa / rmtF / ARR-3 / blaNDM-1 - 1..193462 193462
- flank IS/Tn - - 123741..125009 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T152761 WP_001044770.1 NZ_CP050374:c118594-118178 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T152761 NZ_CP066501:479413-479763 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT152761 WP_001261282.1 NZ_CP050374:c118821-118591 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT152761 NZ_CP066501:479126-479407 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References