Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 49361..50097 Replicon plasmid p50595_NDM_1
Accession NZ_CP050374
Organism Klebsiella pneumoniae strain 50595

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HB637_RS28480 Protein ID WP_003026803.1
Coordinates 49361..49843 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HB637_RS28485 Protein ID WP_003026799.1
Coordinates 49831..50097 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB637_RS28465 44998..45960 + 963 WP_004152113.1 M48 family metalloprotease -
HB637_RS28470 46048..47302 + 1255 Protein_60 IS3 family transposase -
HB637_RS28475 47803..49149 - 1347 WP_020314316.1 ISNCY family transposase -
HB637_RS28480 49361..49843 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HB637_RS28485 49831..50097 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HB637_RS28490 50273..50527 - 255 WP_004152108.1 hypothetical protein -
HB637_RS28495 50603..50860 - 258 WP_004152107.1 hypothetical protein -
HB637_RS28500 50909..51112 - 204 WP_004152106.1 hemolysin expression modulator Hha -
HB637_RS28505 51146..51514 - 369 WP_004152105.1 hypothetical protein -
HB637_RS28510 51558..52052 - 495 WP_004152104.1 DNA-binding protein -
HB637_RS28515 52083..52658 - 576 WP_004152103.1 hypothetical protein -
HB637_RS28520 52646..52915 - 270 WP_004152102.1 hypothetical protein -
HB637_RS28525 53273..53623 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
HB637_RS28530 53673..54035 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / aac(3)-IIa / rmtF / ARR-3 / blaNDM-1 - 1..193462 193462


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T152760 WP_003026803.1 NZ_CP050374:c49843-49361 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T152760 NZ_CP066492:c2512498-2512232 [Staphylococcus aureus]
ATGAGCAATTATACGGTTAAGATTAAAAATTCGGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAAAAGCC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACTGTAGATGATCGAAATAAAGAAGTATTAATA
TTATCGGCGTGGTCACATTATGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT152760 WP_003026799.1 NZ_CP050374:c50097-49831 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT152760 NZ_CP066492:c2512755-2512498 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGATGATTGGAAAAGTATACAAGAAACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGATAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References