Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 19073..19596 Replicon plasmid p50595_NDM_1
Accession NZ_CP050374
Organism Klebsiella pneumoniae strain 50595

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag HB637_RS28290 Protein ID WP_015632464.1
Coordinates 19291..19596 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag HB637_RS28285 Protein ID WP_015632465.1
Coordinates 19073..19291 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB637_RS28260 15216..16487 - 1272 WP_016162068.1 Y-family DNA polymerase -
HB637_RS28265 16487..16918 - 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
HB637_RS28275 17082..17567 - 486 Protein_21 IS5 family transposase -
HB637_RS28280 17862..18887 - 1026 WP_015632466.1 IS110 family transposase -
HB637_RS28285 19073..19291 + 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HB637_RS28290 19291..19596 + 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
HB637_RS28295 19741..20085 + 345 WP_015632463.1 hypothetical protein -
HB637_RS28300 20127..20693 + 567 WP_015632462.1 hypothetical protein -
HB637_RS28305 20897..21154 - 258 WP_016162071.1 helix-turn-helix domain-containing protein -
HB637_RS28310 21584..21811 + 228 WP_016162072.1 hypothetical protein -
HB637_RS28315 21825..21947 + 123 Protein_29 transcriptional regulator -
HB637_RS28320 22153..22596 - 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
HB637_RS28325 22622..22804 - 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
HB637_RS28330 23165..24145 + 981 WP_015632457.1 partitioning protein -
HB637_RS28335 24138..24566 + 429 WP_015632456.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / aac(3)-IIa / rmtF / ARR-3 / blaNDM-1 - 1..193462 193462


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T152759 WP_015632464.1 NZ_CP050374:19291-19596 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T152759 NZ_CP066492:c2481397-2481290 [Staphylococcus aureus]
ATCTTCAATTTATTAATTGACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCAAATTGGTTACGAAC
GCGCAGCAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT152759 WP_015632465.1 NZ_CP050374:19073-19291 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT152759 NZ_CP066492:2481213-2481273 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References