152758

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7990..8633 Replicon plasmid p50595_NDM_1
Accession NZ_CP050374
Organism Klebsiella pneumoniae strain 50595

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HB637_RS28205 Protein ID WP_001044770.1
Coordinates 7990..8406 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HB637_RS28210 Protein ID WP_001261282.1
Coordinates 8403..8633 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB637_RS28185 4908..5240 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
HB637_RS28190 5287..6162 - 876 WP_000239590.1 class A extended-spectrum beta-lactamase CTX-M-15 -
HB637_RS28195 6475..7172 - 698 Protein_6 IS1 family transposase -
HB637_RS28200 7206..7916 - 711 WP_004152334.1 ATP-binding protein -
HB637_RS28205 7990..8406 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HB637_RS28210 8403..8633 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HB637_RS28215 8590..9051 + 462 WP_165763053.1 hypothetical protein -
HB637_RS28220 9196..9510 + 315 WP_015632378.1 hypothetical protein -
HB637_RS28225 9559..9870 + 312 WP_024191724.1 hypothetical protein -
HB637_RS28230 9935..10939 + 1005 WP_016162067.1 hypothetical protein -
HB637_RS28235 10983..11144 + 162 WP_032495756.1 hypothetical protein -
HB637_RS28240 11137..11931 + 795 WP_015632375.1 site-specific integrase -
HB637_RS28245 12379..12558 - 180 WP_006788217.1 hypothetical protein -
HB637_RS28250 12678..13304 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / aac(3)-IIa / rmtF / ARR-3 / blaNDM-1 - 1..193462 193462


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T152758 WP_001044770.1 NZ_CP050374:c8406-7990 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T152758 NZ_CP066492:c2456231-2455965 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT152758 WP_001261282.1 NZ_CP050374:c8633-8403 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT152758 NZ_CP066492:c2456482-2456231 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References