Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 123178..123923 Replicon plasmid p50595_IncFII
Accession NZ_CP050373
Organism Klebsiella pneumoniae strain 50595

Toxin (Protein)


Gene name tacT Uniprot ID A0A331KSM6
Locus tag HB637_RS28100 Protein ID WP_032408901.1
Coordinates 123178..123669 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag HB637_RS28105 Protein ID WP_014386183.1
Coordinates 123657..123923 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB637_RS28075 118862..119617 + 756 WP_032408898.1 DeoR/GlpR family DNA-binding transcription regulator -
HB637_RS28080 119879..120319 + 441 WP_014386179.1 IS66 family insertion sequence hypothetical protein -
HB637_RS28085 120316..120666 + 351 WP_014386180.1 IS66 family insertion sequence element accessory protein TnpB -
HB637_RS28090 120697..122289 + 1593 Protein_135 IS66 family transposase -
HB637_RS28095 122486..122737 + 252 WP_032408900.1 aldo/keto reductase -
HB637_RS28100 123178..123669 - 492 WP_032408901.1 GNAT family N-acetyltransferase Toxin
HB637_RS28105 123657..123923 - 267 WP_014386183.1 DUF1778 domain-containing protein Antitoxin
HB637_RS28110 124120..124278 + 159 WP_014386184.1 type I toxin-antitoxin system Hok family toxin -
HB637_RS28115 124522..125007 - 486 WP_014386185.1 hypothetical protein -
HB637_RS28120 125197..125469 + 273 WP_032408902.1 hypothetical protein -
HB637_RS28125 125466..125816 + 351 WP_014386186.1 hypothetical protein -
HB637_RS28140 126453..126779 + 327 WP_014386187.1 hypothetical protein -
HB637_RS28145 126841..127053 + 213 WP_014386188.1 hypothetical protein -
HB637_RS28150 127064..127288 + 225 WP_014386189.1 hypothetical protein -
HB637_RS28155 127369..127683 + 315 Protein_146 type II toxin-antitoxin system RelE/ParE family toxin -
HB637_RS28160 127737..127925 - 189 WP_000330527.1 DDE-type integrase/transposase/recombinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..127925 127925
- flank IS/Tn - - 127737..127868 131


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(89-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17667.46 Da        Isoelectric Point: 8.8757

>T152757 WP_032408901.1 NZ_CP050373:c123669-123178 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Download         Length: 492 bp

>T152757 NZ_CP066492:c2203073-2202969 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.66 Da        Isoelectric Point: 5.1169

>AT152757 WP_014386183.1 NZ_CP050373:c123923-123657 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Download         Length: 267 bp

>AT152757 NZ_CP066492:2202876-2202914 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331KSM6

Antitoxin

Source ID Structure

References