Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 56596..57239 Replicon plasmid p47733_CTX_M_15
Accession NZ_CP050364
Organism Klebsiella pneumoniae strain 47733

Toxin (Protein)


Gene name vagD Uniprot ID R9WRW3
Locus tag HB636_RS27750 Protein ID WP_015063455.1
Coordinates 56823..57239 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HB636_RS27745 Protein ID WP_001261276.1
Coordinates 56596..56826 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB636_RS27710 51710..51979 + 270 WP_000339857.1 hypothetical protein -
HB636_RS27715 52156..53022 - 867 WP_004118283.1 replication initiation protein -
HB636_RS27720 53557..53661 + 105 WP_032409716.1 hypothetical protein -
HB636_RS27725 53790..54047 + 258 WP_000764642.1 hypothetical protein -
HB636_RS27730 54105..54881 - 777 WP_000015958.1 site-specific integrase -
HB636_RS27735 54878..55621 - 744 WP_000129823.1 hypothetical protein -
HB636_RS27740 55672..56022 - 351 WP_000493378.1 hypothetical protein -
HB636_RS27745 56596..56826 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HB636_RS27750 56823..57239 + 417 WP_015063455.1 type II toxin-antitoxin system VapC family toxin Toxin
HB636_RS27755 57483..58339 + 857 Protein_52 IS3-like element ISEc15 family transposase -
HB636_RS27760 58621..59697 + 1077 WP_000227969.1 IS110 family transposase -
HB636_RS27765 59714..59992 + 279 Protein_54 IS3 family transposase -
HB636_RS27770 60295..61851 - 1557 WP_053897648.1 PAS domain-containing methyl-accepting chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrB1 / dfrA14 / catA2 / mph(A) / rmtF / dfrA12 / blaCTX-M-15 - 1..119981 119981


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15036.50 Da        Isoelectric Point: 8.5440

>T152730 WP_015063455.1 NZ_CP050364:56823-57239 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVN

Download         Length: 417 bp

>T152730 NZ_CP066469:3932-4189 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT152730 WP_001261276.1 NZ_CP050364:56596-56826 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT152730 NZ_CP066469:3670-3939 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R9WRW3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References