152729

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 47077..47600 Replicon plasmid p47733_ARR2
Accession NZ_CP050361
Organism Klebsiella pneumoniae strain 47733

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag HB636_RS27125 Protein ID WP_015632464.1
Coordinates 47295..47600 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag HB636_RS27120 Protein ID WP_015632465.1
Coordinates 47077..47295 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB636_RS27095 43220..44491 - 1272 WP_016162068.1 Y-family DNA polymerase -
HB636_RS27100 44491..44922 - 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
HB636_RS27110 45086..45571 - 486 Protein_48 IS5 family transposase -
HB636_RS27115 45866..46891 - 1026 WP_015632466.1 IS110 family transposase -
HB636_RS27120 47077..47295 + 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HB636_RS27125 47295..47600 + 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
HB636_RS27130 47745..48089 + 345 WP_015632463.1 hypothetical protein -
HB636_RS27135 48131..48697 + 567 WP_015632462.1 hypothetical protein -
HB636_RS27140 48901..49158 - 258 WP_016162071.1 helix-turn-helix domain-containing protein -
HB636_RS27145 49588..49815 + 228 WP_016162072.1 hypothetical protein -
HB636_RS27150 49829..49951 + 123 Protein_56 transcriptional regulator -
HB636_RS27155 50157..50600 - 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
HB636_RS27160 50626..50808 - 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
HB636_RS27165 51169..52149 + 981 WP_015632457.1 partitioning protein -
HB636_RS27170 52142..52570 + 429 WP_015632456.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid rmtF / ARR-3 - 1..107451 107451
- inside Integron - - 2..107449 107447


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T152729 WP_015632464.1 NZ_CP050361:47295-47600 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T152729 NZ_CP066467:17069-17191 [Enterococcus faecium]
ATGAAAGGAGTAAGCCTTTTGTCCGCATATGAGACAATTCAAACCATTCTTGGTTTTGGTATGTTTACCATTGCTTTGAT
TAGTTTGATTGTAAATATGCTTAAAAATGACAAAAAGAAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT152729 WP_015632465.1 NZ_CP050361:47077-47295 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT152729 NZ_CP066467:c17274-17173 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References