Detailed information of TA system TA152725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4023579..4025260
• Replicon: chromosome
• Accession: NZ_CP050360
• Organism: Klebsiella pneumoniae strain 47733

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A6TH06
• Locus tag: HB636_RS20150
• Protein ID: WP_015959269.1
• Coordinates: 4024385..4025260 (+)
• Length: 292 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: HB636_RS20145
• Protein ID: WP_004177809.1
• Coordinates: 4023579..4024337 (+)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB636_RS20135	4020345..4022492	-	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
HB636_RS20140	4022765..4023493	+	729	WP_032413197.1	response regulator transcription factor	-
HB636_RS20145	4023579..4024337	+	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
HB636_RS20150	4024385..4025260	+	876	WP_015959269.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HB636_RS20155	4025385..4026392	-	1008	WP_015959270.1	carbohydrate kinase family protein	-
HB636_RS20160	4026467..4027408	-	942	WP_002885156.1	ABC transporter substrate-binding protein	-
HB636_RS20165	4027439..4028437	-	999	WP_002885172.1	ribose ABC transporter permease	-
HB636_RS20170	4028434..4029954	-	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (7-264)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 292 a.a.
• Molecular weight: 32990.17 Da
• Isoelectric Point: 5.4637

>T152725 WP_015959269.1 NZ_CP050360:4024385-4025260 [Klebsiella pneumoniae]
MQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQAGMSAVLQANRSDE
MRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQHPRDFYDVKLLLDAQ
ELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKSCITQQDVDFLLSFK
RGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 876 bp

>T152725 NZ_CP066460:c14335-13985 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT152725 WP_004177809.1 NZ_CP050360:4023579-4024337 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT152725 NZ_CP066460:c14592-14329 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A6TH06

Antitoxin

• AlphaFold DB: A6TH05