Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2097531..2098267 Replicon chromosome
Accession NZ_CP050360
Organism Klebsiella pneumoniae strain 47733

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag HB636_RS10475 Protein ID WP_032433360.1
Coordinates 2097531..2098013 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HB636_RS10480 Protein ID WP_003026799.1
Coordinates 2098001..2098267 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB636_RS10455 2094080..2094820 + 741 WP_050597964.1 molecular chaperone -
HB636_RS10460 2094824..2095402 + 579 WP_032432061.1 type 1 fimbrial protein -
HB636_RS10465 2095414..2096358 + 945 WP_077254249.1 fimbrial protein -
HB636_RS10470 2096571..2097170 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
HB636_RS10475 2097531..2098013 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
HB636_RS10480 2098001..2098267 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HB636_RS10485 2098580..2099143 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
HB636_RS10490 2099153..2099785 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
HB636_RS10495 2099803..2100807 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
HB636_RS10500 2100791..2101453 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
HB636_RS10505 2101482..2102621 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2085811..2130322 44511


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T152719 WP_032433360.1 NZ_CP050360:c2098013-2097531 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T152719 NZ_CP066451:515440-515781 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT152719 WP_003026799.1 NZ_CP050360:c2098267-2098001 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT152719 NZ_CP066451:515210-515440 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References