Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 23012..23655 Replicon plasmid unnamed3
Accession NZ_CP050357
Organism Klebsiella pneumoniae strain ZRKP01

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HB786_RS28750 Protein ID WP_001044770.1
Coordinates 23239..23655 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HB786_RS28745 Protein ID WP_001261282.1
Coordinates 23012..23242 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB786_RS28710 18235..19014 - 780 WP_013214009.1 site-specific integrase -
HB786_RS28715 19198..20202 - 1005 WP_011977814.1 hypothetical protein -
HB786_RS28720 20232..20435 - 204 WP_011977813.1 hypothetical protein -
HB786_RS28725 20481..21002 - 522 WP_094960442.1 hypothetical protein -
HB786_RS28730 21060..21452 - 393 WP_011977811.1 hypothetical protein -
HB786_RS28735 21489..22433 - 945 WP_011977810.1 hypothetical protein -
HB786_RS28740 22594..23055 - 462 WP_014343465.1 hypothetical protein -
HB786_RS28745 23012..23242 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HB786_RS28750 23239..23655 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HB786_RS28755 23729..25291 + 1563 WP_004206609.1 AAA family ATPase -
HB786_RS28760 25276..26298 + 1023 WP_000361404.1 helicase UvrD -
HB786_RS28765 26554..27251 + 698 Protein_37 IS1 family transposase -
HB786_RS28770 27280..27552 + 273 Protein_38 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-65 / blaKPC-2 / catA2 - 1..91097 91097


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T152707 WP_001044770.1 NZ_CP050357:23239-23655 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T152707 NZ_CP066429:37385-37642 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT152707 WP_001261282.1 NZ_CP050357:23012-23242 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT152707 NZ_CP066429:37123-37392 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References