Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5357638..5358263 Replicon chromosome
Accession NZ_CP050354
Organism Klebsiella pneumoniae strain ZRKP01

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HB786_RS26345 Protein ID WP_002882817.1
Coordinates 5357638..5358021 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HB786_RS26350 Protein ID WP_004150355.1
Coordinates 5358021..5358263 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB786_RS26330 5355004..5355906 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HB786_RS26335 5355903..5356538 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HB786_RS26340 5356535..5357464 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HB786_RS26345 5357638..5358021 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HB786_RS26350 5358021..5358263 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HB786_RS26355 5358468..5359385 + 918 WP_002882812.1 alpha/beta hydrolase -
HB786_RS26360 5359399..5360340 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HB786_RS26365 5360385..5360822 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HB786_RS26370 5360819..5361679 - 861 WP_002882807.1 virulence factor BrkB family protein -
HB786_RS26375 5361673..5362272 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T152705 WP_002882817.1 NZ_CP050354:c5358021-5357638 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T152705 NZ_CP066427:218356-218622 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT152705 WP_004150355.1 NZ_CP050354:c5358263-5358021 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT152705 NZ_CP066427:218093-218356 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References