Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4161420..4162039 Replicon chromosome
Accession NZ_CP050354
Organism Klebsiella pneumoniae strain ZRKP01

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HB786_RS20615 Protein ID WP_002892050.1
Coordinates 4161821..4162039 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HB786_RS20610 Protein ID WP_002892066.1
Coordinates 4161420..4161794 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB786_RS20600 4156572..4157765 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HB786_RS20605 4157788..4160934 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
HB786_RS20610 4161420..4161794 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HB786_RS20615 4161821..4162039 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HB786_RS20620 4162198..4162764 + 567 WP_002892030.1 maltose O-acetyltransferase -
HB786_RS20625 4162736..4162876 - 141 WP_004147370.1 hypothetical protein -
HB786_RS20630 4162897..4163367 + 471 WP_002892026.1 YlaC family protein -
HB786_RS20635 4163342..4164793 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HB786_RS20640 4164894..4165592 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
HB786_RS20645 4165589..4165729 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HB786_RS20650 4165729..4165992 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T152701 WP_002892050.1 NZ_CP050354:4161821-4162039 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T152701 NZ_CP066421:198240-198506 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGACAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAGAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT152701 WP_002892066.1 NZ_CP050354:4161420-4161794 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT152701 NZ_CP066421:197977-198240 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACAGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References