Detailed information of TA system TA152687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 45..519
• Replicon: plasmid unnamed5
• Accession: NZ_CP050353
• Organism: Klebsiella pneumoniae strain ZRKP02

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: HB787_RS29200
• Protein ID: WP_004197762.1
• Coordinates: 253..519 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: HB787_RS29195
• Protein ID: WP_000879771.1
• Coordinates: 45..269 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB787_RS29195	45..269	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
HB787_RS29200	253..519	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB787_RS29205	589..939	-	351	WP_004197756.1	hypothetical protein	-
HB787_RS29210	1005..1550	-	546	WP_023317533.1	hypothetical protein	-
HB787_RS29215	1576..1955	-	380	Protein_4	hypothetical protein	-
HB787_RS29220	3058..3234	+	177	WP_004197757.1	hypothetical protein	-
HB787_RS29225	3224..3568	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
HB787_RS29230	3507..4271	+	765	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
HB787_RS29235	4246..4755	+	510	WP_032433972.1	MbeB family mobilization protein	-
HB787_RS29240	4762..4974	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T152687 WP_004197762.1 NZ_CP050353:253-519 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T152687 NZ_CP066390:c126353-126081 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT152687 WP_000879771.1 NZ_CP050353:45-269 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT152687 NZ_CP066390:c126616-126353 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5