Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 52671..53314 Replicon plasmid unnamed3
Accession NZ_CP050351
Organism Klebsiella pneumoniae strain ZRKP02

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HB787_RS28855 Protein ID WP_001044770.1
Coordinates 52671..53087 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HB787_RS28860 Protein ID WP_001261282.1
Coordinates 53084..53314 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB787_RS28835 48774..49046 - 273 Protein_65 transposase -
HB787_RS28840 49075..49772 - 698 Protein_66 IS1 family transposase -
HB787_RS28845 50028..51050 - 1023 WP_000361404.1 helicase UvrD -
HB787_RS28850 51035..52597 - 1563 WP_004206609.1 AAA family ATPase -
HB787_RS28855 52671..53087 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HB787_RS28860 53084..53314 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HB787_RS28865 53271..53732 + 462 WP_014343465.1 hypothetical protein -
HB787_RS28870 53893..54837 + 945 WP_011977810.1 hypothetical protein -
HB787_RS28875 54874..55266 + 393 WP_011977811.1 hypothetical protein -
HB787_RS28880 55324..55845 + 522 WP_094960442.1 hypothetical protein -
HB787_RS28885 55891..56094 + 204 WP_011977813.1 hypothetical protein -
HB787_RS28890 56124..57128 + 1005 WP_011977814.1 hypothetical protein -
HB787_RS28895 57312..58091 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-33 / blaCTX-M-65 / catA2 - 1..91502 91502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T152681 WP_001044770.1 NZ_CP050351:c53087-52671 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T152681 NZ_CP066381:521527-521868 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT152681 WP_001261282.1 NZ_CP050351:c53314-53084 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT152681 NZ_CP066381:521297-521527 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References