Detailed information of TA system TA152661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 4805..5385
• Replicon: plasmid unnamed4
• Accession: NZ_CP050346
• Organism: Klebsiella pneumoniae strain ZRKP03

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: HB788_RS29035
• Protein ID: WP_071177730.1
• Coordinates: 4805..5119 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: HB788_RS29040
• Protein ID: WP_000093040.1
• Coordinates: 5107..5385 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB788_RS29010	749..2713	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
HB788_RS29015	2713..3444	+	732	WP_071177728.1	replication-relaxation family protein	-
HB788_RS29020	3451..3981	+	531	WP_071177729.1	hypothetical protein	-
HB788_RS29025	4008..4187	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
HB788_RS29030	4213..4641	-	429	WP_001140599.1	hypothetical protein	-
HB788_RS29035	4805..5119	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB788_RS29040	5107..5385	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
HB788_RS29045	5719..5925	-	207	Protein_7	TonB family protein	-
HB788_RS29050	5922..6293	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
HB788_RS29055	6567..6812	-	246	WP_032440458.1	hypothetical protein	-
HB788_RS29060	7139..8824	+	1686	WP_032440457.1	cloacin	-
HB788_RS29065	8834..9091	+	258	WP_032440455.1	cloacin	-
HB788_RS29070	9176..9325	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T152661 WP_071177730.1 NZ_CP050346:c5119-4805 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T152661 NZ_CP066369:1264966-1265406 [Shewanella putrefaciens]
GTGGAAGAGCGGCACCATAGTTTTAGTGTTAAAGTCTCCTTCGACCAACGACTCTCGTTGGTCGTTTTTATGTCTATTTG
CCTATCATCCTTGCTCGCTTGGCCCCAAACAGAATCCTTTGGCGTTTTTCTATTAAAATTGGCGGTTATTTCGCTGATTG
TGTGTTTTTTGCTGTATCAACTTTGGCGACTTAAAGATTGGCAATTCAGTTTTGTACTCAATCGAAATGGCGAGGGGCGG
CTTGCTAGTGGGGAGCATTTTCAGGTTTTGCGGCGCACTTGGGTGACCCCGTTTGTATGTTTGATGTATCTTGATATCGA
TACTAAACCGCGGTTATTACCGCTTTGGGCCGATATGTTTACCGATGCCGATTATCGGCATTTATGTCGCTTGCTGCTCA
ATGCTAAAACATTGCAGGCTAAATCTCGTACTGAAATTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT152661 WP_000093040.1 NZ_CP050346:c5385-5107 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT152661 NZ_CP066369:1264737-1264985 [Shewanella putrefaciens]
TTGGAATTAATGAACATTGCACGGGTGCGTTGGGCATGTCGCCGCGGGATGCTGGAGCTAGACGTACTGTTTCAACCCTT
CGTCGAACATGTTTATCAAGAGTTGTCCGATGAAGATAAAGCCGTGTTTATTCGCTTACTCGAGTGTGAAGATCCGGAAT
TATTTGCGTGGTTTATGGGCCATGAAACCTGCCCAGACGCTGAGCTAGCTCGTATGGTAGTTAAAGTCCGTGGAAGAGCG
GCACCATAG

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1