Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5356497..5357122 Replicon chromosome
Accession NZ_CP050342
Organism Klebsiella pneumoniae strain ZRKP03

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HB788_RS26335 Protein ID WP_002882817.1
Coordinates 5356497..5356880 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HB788_RS26340 Protein ID WP_004150355.1
Coordinates 5356880..5357122 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB788_RS26320 5353863..5354765 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HB788_RS26325 5354762..5355397 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HB788_RS26330 5355394..5356323 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HB788_RS26335 5356497..5356880 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HB788_RS26340 5356880..5357122 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HB788_RS26345 5357327..5358244 + 918 WP_002882812.1 alpha/beta hydrolase -
HB788_RS26350 5358258..5359199 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HB788_RS26355 5359244..5359681 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HB788_RS26360 5359678..5360538 - 861 WP_002882807.1 virulence factor BrkB family protein -
HB788_RS26365 5360532..5361131 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T152656 WP_002882817.1 NZ_CP050342:c5356880-5356497 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T152656 NZ_CP066368:12390-12650 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT152656 WP_004150355.1 NZ_CP050342:c5357122-5356880 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT152656 NZ_CP066368:12640-12891 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References