Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 18168..18811 Replicon plasmid unnamed3
Accession NZ_CP050339
Organism Klebsiella pneumoniae strain ZRKP04

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HB789_RS28730 Protein ID WP_001044770.1
Coordinates 18395..18811 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HB789_RS28725 Protein ID WP_001261282.1
Coordinates 18168..18398 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB789_RS28690 13391..14170 - 780 WP_013214009.1 site-specific integrase -
HB789_RS28695 14354..15358 - 1005 WP_011977814.1 hypothetical protein -
HB789_RS28700 15388..15591 - 204 WP_011977813.1 hypothetical protein -
HB789_RS28705 15637..16158 - 522 WP_094960442.1 hypothetical protein -
HB789_RS28710 16216..16608 - 393 WP_011977811.1 hypothetical protein -
HB789_RS28715 16645..17589 - 945 WP_011977810.1 hypothetical protein -
HB789_RS28720 17750..18211 - 462 WP_014343465.1 hypothetical protein -
HB789_RS28725 18168..18398 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HB789_RS28730 18395..18811 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HB789_RS28735 18885..20447 + 1563 WP_004206609.1 AAA family ATPase -
HB789_RS28740 20432..21454 + 1023 WP_000361404.1 helicase UvrD -
HB789_RS28745 21710..22407 + 698 Protein_31 IS1 family transposase -
HB789_RS28750 22436..22708 + 273 Protein_32 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 / catA2 - 1..86253 86253


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T152635 WP_001044770.1 NZ_CP050339:18395-18811 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T152635 NZ_CP066366:821069-821476 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT152635 WP_001261282.1 NZ_CP050339:18168-18398 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT152635 NZ_CP066366:820822-821088 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References