152633

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5359067..5359692 Replicon chromosome
Accession NZ_CP050336
Organism Klebsiella pneumoniae strain ZRKP04

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HB789_RS26355 Protein ID WP_002882817.1
Coordinates 5359067..5359450 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HB789_RS26360 Protein ID WP_004150355.1
Coordinates 5359450..5359692 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB789_RS26340 5356433..5357335 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HB789_RS26345 5357332..5357967 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HB789_RS26350 5357964..5358893 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HB789_RS26355 5359067..5359450 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HB789_RS26360 5359450..5359692 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HB789_RS26365 5359897..5360814 + 918 WP_002882812.1 alpha/beta hydrolase -
HB789_RS26370 5360828..5361769 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HB789_RS26375 5361814..5362251 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HB789_RS26380 5362248..5363108 - 861 WP_002882807.1 virulence factor BrkB family protein -
HB789_RS26385 5363102..5363701 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T152633 WP_002882817.1 NZ_CP050336:c5359450-5359067 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T152633 NZ_CP066366:649046-649360 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT152633 WP_004150355.1 NZ_CP050336:c5359692-5359450 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT152633 NZ_CP066366:649357-649773 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References