Detailed information of TA system TA152614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5245777..5246417
• Replicon: chromosome
• Accession: NZ_CP050335
• Organism: Pseudomonas aeruginosa strain DVT401

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HB725_RS24415
• Protein ID: WP_003105740.1
• Coordinates: 5245777..5246187 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: HB725_RS24420
• Protein ID: WP_003158175.1
• Coordinates: 5246187..5246417 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB725_RS24375	5241619..5241816	+	198	WP_003105756.1	hypothetical protein	-
HB725_RS24380	5241972..5242700	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
HB725_RS24385	5242706..5243254	+	549	WP_003105753.1	DUF3158 family protein	-
HB725_RS24390	5243301..5244140	+	840	WP_003105750.1	Rha family transcriptional regulator	-
HB725_RS24395	5244170..5244658	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
HB725_RS24400	5244766..5245011	+	246	WP_023102333.1	CrpP family protein	-
HB725_RS24405	5245077..5245295	-	219	WP_003105747.1	hypothetical protein	-
HB725_RS24410	5245495..5245755	-	261	WP_003105742.1	hypothetical protein	-
HB725_RS24415	5245777..5246187	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HB725_RS24420	5246187..5246417	-	231	WP_003158175.1	antitoxin	Antitoxin
HB725_RS24425	5246673..5248592	+	1920	WP_004352838.1	type I DNA topoisomerase	-
HB725_RS24430	5248900..5249109	+	210	WP_003105733.1	cold-shock protein	-
HB725_RS24435	5249330..5251219	+	1890	WP_003105732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5226206..5363049	136843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T152614 WP_003105740.1 NZ_CP050335:c5246187-5245777 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T152614 NZ_CP066358:c1019936-1019382 [Sinorhizobium meliloti]
ATGAGGAAACACATTCCGCCCGCATCGCCACCCGATCCGGAACCGACGTCGCCGGTCGAGCTGATAACGCTCTGGCGCGC
CTTCGTTCCGCGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCCGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGCA
TGCCTGCGATTTATGCTGCGCGCGAGCTTTCCACTGCCTGGGCTGAATATAATCAGGGTTTCGTGCAGCATCCGGCGCTC
ATCGTGCAGCTCGAGTTGCGCGGGGCGAGGCTCGCGGACCTCACGGATGCCAGCGTTCTTCTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAAGGCGCGGTGCCGGAGACCCACAGGCTGCAGTCGGAGCTTC
TGGCGCGCGACTATCACGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGCGTCGCGCTCTGGCGCTGGAAC
GGTGCGGAAGAACCGCGTCTCGACGTCATCGATCCCGATGGCCGGCTGCCGAAATCGCCCGCCTCATGGGTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT152614 WP_003158175.1 NZ_CP050335:c5246417-5246187 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT152614 NZ_CP066358:c1020314-1019943 [Sinorhizobium meliloti]
ATGATGGCCGTTGATTTCCAGATACCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTTGTCGC
CGACCGGTTGGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTCGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTCGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATCCCCGGCTGGGCCGGCAAGACGGCGTTCGATCTGGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGTGCCGGCGTCTACGCTTAA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2