Detailed information of TA system TA152613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4854905..4855513
• Replicon: chromosome
• Accession: NZ_CP050335
• Organism: Pseudomonas aeruginosa strain DVT401

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: HB725_RS22605
• Protein ID: WP_033979326.1
• Coordinates: 4854905..4855252 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: HB725_RS22610
• Protein ID: WP_003114155.1
• Coordinates: 4855262..4855513 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB725_RS22575	4850265..4850537	+	273	WP_003120794.1	cysteine-rich CWC family protein	-
HB725_RS22580	4850537..4851229	+	693	WP_003114159.1	16S rRNA pseudouridine(516) synthase	-
HB725_RS22585	4851365..4852408	+	1044	WP_003098363.1	L,D-transpeptidase	-
HB725_RS22590	4852488..4853225	+	738	WP_023112775.1	murein L,D-transpeptidase catalytic domain family protein	-
HB725_RS22595	4853677..4854579	+	903	WP_023112774.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
HB725_RS22605	4854905..4855252	-	348	WP_033979326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB725_RS22610	4855262..4855513	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HB725_RS22615	4855727..4856710	-	984	WP_033979328.1	tyrosine-type recombinase/integrase	-
HB725_RS22620	4856710..4858002	-	1293	WP_003115206.1	hypothetical protein	-
HB725_RS22625	4858232..4859506	-	1275	WP_033979330.1	zonular occludens toxin family protein	-
HB725_RS22630	4859510..4859866	-	357	WP_003114150.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	4854905..4879153	24248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13018.79 Da
• Isoelectric Point: 4.4212

>T152613 WP_033979326.1 NZ_CP050335:c4855252-4854905 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T152613 NZ_CP066357:186907-187203 [Bradyrhizobium elkanii USDA 76]
GTGAAACTCGTCTGGTCGCGCTTCGCGTTGTCCGATCGCGACAGCATCTTCAGCTACATCGAGGCCGAGAATCCTCGTGC
CGCGGTCCATGTCGACGAACAGATTGCGAATGCCGCGCGGCGCCTGCTCGACTTTCCGGATAGCGGTCGGCCGGGCCGTG
TCGCTGGGACGCGCGAGTTGGTCATTCCGCGTACATCTTATGTCGCGGCGTACCTGGTCGACGGCGATACCGTTCGCATC
TTGCGCGTGCTCCATGGCGCGCAGATGTGGCCTGACGAACTTACCAACGACGATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT152613 WP_003114155.1 NZ_CP050335:c4855513-4855262 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT152613 NZ_CP066357:186602-186910 [Bradyrhizobium elkanii USDA 76]
ATGGCTTCGAATGCCTTGGTCCAAACCCGCATCGATGCGGACGTGAAGGAAAAGGCCACTGCGGTTCTGGAAAATATGGG
TCTCACGGTATCCGACGCCGTGCGGATCCTGCTGACGCGCACGGCCAATGAAGGGATGCTTCCGCTGGAGCTCGTGAGCA
ACAGCCAAGCTTACGATACCTGGTTTCGTGAGAAGGTGCGCCAGGCGTTGGCCGACACGCGGCCTGATATCGATGATTCC
GAGGTCGAAGCGCATTTCGCACAACGCCGTGCCGCTGCGCTTCGAAAAGCGCCGGAGCAGGAACGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355