Detailed information of TA system TA152611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4463068..4464326
• Replicon: chromosome
• Accession: NZ_CP050335
• Organism: Pseudomonas aeruginosa strain DVT401

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HB725_RS20690
• Protein ID: WP_003163133.1
• Coordinates: 4463068..4463823 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HB725_RS20695
• Protein ID: WP_003086329.1
• Coordinates: 4463958..4464326 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB725_RS20680	4458245..4460788	+	2544	WP_174816558.1	acyl-homoserine-lactone acylase QuiP	-
HB725_RS20685	4460944..4462305	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
HB725_RS20690	4463068..4463823	-	756	WP_003163133.1	RES family NAD+ phosphorylase	Toxin
HB725_RS20695	4463958..4464326	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HB725_RS20700	4464399..4465685	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
HB725_RS20705	4465741..4467330	-	1590	WP_078452049.1	aldehyde dehydrogenase family protein	-
HB725_RS20710	4467409..4467885	+	477	WP_003112531.1	hypothetical protein	-
HB725_RS20715	4467888..4468163	-	276	WP_010792073.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T152611 WP_003163133.1 NZ_CP050335:c4463823-4463068 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T152611 NZ_CP066355:c8256712-8255789 [Bradyrhizobium diazoefficiens]
ATGATCGGCAAGCCGCTCAAAGATCCCGGTGCTTCGATCCGTCAGCGCCTGCTCAACCACGCCAAGGAGCACGGCGACGA
GTATCAACGGATTCTGACGCGCTACGCGATTGAGCGGTTGCTTTTCAGACTAAGCCAAACCGAGGCGCGCGAGCGATACG
TCCTCAAGGGCGCGATGCTCTTCGTGACTTGGCCCGAACAAGTGTTTCGGCCCACAGGTGATCTTGACCTTCTTGGGCAA
GGGGATCCCGATCCAGCTGCCATCATCGATCTCTTCACCCTTATCTGCCAGGTCGAGGCGCCGGCGGATGGCATCATCTT
CGACCCCTCTACGCTGCAGGTGGAGGCGGTCCGCGAGGAGGAAAAATATCAGGGTGTCAGACTCTCCCTGAGGGGACGTC
TTGGCACGGCGGTGATCCCTGTCCAGGTGGACATCGGGTTTGGGGACAGTGTTCATCCCGCGCCCAGCCGGAACAATTTC
CCCAGCCTGCTGCCGGACCTGCCGGCGGCGGACGTCCTGATGTACCCGCCCGAGACAGTAATTGCAGAGAAATTCGAAGC
GATGATCCGCTTTGGCGAAGCGAACGGCAGGATCAAAGATTTTCACGATATCTGGGTCACGGCGCGAACCTTCGCTTTCA
ACCTCTCTACGCTGGTCGAGGCGGTTAGCGGTACCCTGCAAAGGCGGGAAACCGCGTTCCCGACGGAGATGCCGGTGGCG
CTGACGTCTCAGTTCGCCGAGATGGCCGACAAACAAGCGCTATGGACAGGCTTTCTGCGCCGCAATCCGCCCACGCTGCC
GCCGCCTCCATTGGGCGACTTGTTGGCGGAGCTGCGGCGGTTCTTCGAACCCGTGCTTGCGGCTCTTCCTTTGCCGGAAA
GCGCGACCGGTCGTTGGAATCCGGACCGCGGCGCCTGGGAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT152611 WP_003086329.1 NZ_CP050335:c4464326-4463958 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT152611 NZ_CP066355:c8257401-8256709 [Bradyrhizobium diazoefficiens]
ATGACATCAGCTGCTAATCTTCAAATGTTGTTCAATGTCGACAAATCTGCTGACGTGCCGACAAAAGATAACAAAATGAC
CCAACGTCATGTGCTGCCTTATCGCGAGCGCGCCCTCAATCTCGTTCGGGCTCGAGGCATTGCCCGCGCCCGCGACTTCA
AAGCCGCGGGTATCCCCCTTGTTTACCTGAAGCGACTGACAGACACAGGTGAACTCGTTCGACTTGGAAGAGGGCTCTAC
CAAGATCCGGATCGAGTCGGAGAAGATGTCGCTCATAATCTTGCGGAAGCCGCACGGGCCGTTCCCAACGGAGTCGTTAG
CCTTGCCAGTGCGCTCCGGTACCACGGGTTGACGACCCAGCTGCCGCATGCCGTCTGGCTGACCATTCCGCACAAAGCTA
GAACTCCGAAGGCCATTGGCTTCCCGTTGGAAATCGTCCGCGCCACAGGCGATGTGATGACAGCTGGCGTCGAGCATGTG
CAGGTCGAAGGCGTCGACGTCCCCATATACGGCGTAGCTAAAACCATCGCCGATTGCTTCAAGCACCGTCGTCATATCGG
GGAGGACGTCGCGATCGAGGCACTCCGCGATGCGCTCCGCCTGCGGAAGACTACAGTGAGCGATGTCATGAGGTACGCCG
AGATCGATCGTGTGGCCGAGAGAATCCGCCCCGTTCTTAAGGCGCTTCAATGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7