Detailed information of TA system TA152604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1780590..1782098
• Replicon: chromosome
• Accession: NZ_CP050334
• Organism: Pseudomonas aeruginosa strain DVT410

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX1
• Locus tag: HB727_RS08230
• Protein ID: WP_003119682.1
• Coordinates: 1780590..1781177 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX2
• Locus tag: HB727_RS08235
• Protein ID: WP_003115566.1
• Coordinates: 1781244..1782098 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB727_RS08225	1776845..1780324	-	3480	WP_003114080.1	hybrid sensor histidine kinase/response regulator	-
HB727_RS08230	1780590..1781177	+	588	WP_003119682.1	GNAT family N-acetyltransferase	Toxin
HB727_RS08235	1781244..1782098	+	855	WP_003115566.1	AraC family transcriptional regulator	Antitoxin
HB727_RS08240	1782195..1784360	+	2166	WP_174818027.1	TonB-dependent receptor family protein	-
HB727_RS08245	1784535..1786409	+	1875	WP_003091605.1	MFS transporter	-
HB727_RS08250	1786470..1786679	-	210	WP_003091604.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21901.23 Da
• Isoelectric Point: 9.8676

>T152604 WP_003119682.1 NZ_CP050334:1780590-1781177 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T152604 NZ_CP066351:c3268183-3267830 [Bradyrhizobium japonicum]
ATGAAGCGAGGTGAGATCTGGACGGTGGCAGGCGGCAAGGACTATGCGGGCAAGCCGCGCCCCGTCGTCATCGTGCAGGA
CGACAGCTTCGACGCCACGAACTCGATCACTGTCTGCGCTTTCACAACCGACGAAACCGAGGCGCCGCTTTTCCGGCTCA
AGGTCGAGCCAAACGACAACAACGGCCTTCGAGTGCCGTCCCGGCTGATGGTCGATAAGATTACCACGGTGCCGAAAGCC
AAGGTGGGAACGCTAGTCGGTCGCCTAGACGATGACGACCTCTTGCGTCTCAACCGCGCTGTGCTGGTGTTCCTTGGACT
GGCTGGTTCGCAGAGAAGCGCCAGCGCAGATTAG

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31933.23 Da
• Isoelectric Point: 7.3276

>AT152604 WP_003115566.1 NZ_CP050334:1781244-1782098 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 855 bp

>AT152604 NZ_CP066351:c3268401-3268180 [Bradyrhizobium japonicum]
ATGAGCCCTTCAAAGCCCAAGCCGTCTCGTCTGAAGGTGCAGGCCCACCGTGATCGATTGCGCGCACAAGGCCTGCGGCC
GATTCAGATTTGGGTGCCGGATATTCGCTCTCCGTCCTTTCGATCGGAAGCCCATCGCCAATCGCTTGCGGTCGCGACCA
GTGTACATGCATCCGAAGACCAGGGCTTCATCGACGCCGTATCCGATTGGACGGATGAATGA

Structures

Toxin

• PDB: 1YRE
• PDB: 8GXJ

Antitoxin

• AlphaFold DB: Q9HYX2