Detailed information of TA system TA152598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2022815..2024036
• Replicon: chromosome
• Accession: NZ_CP050333
• Organism: Pseudomonas aeruginosa strain DVT412

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: HB729_RS09530
• Protein ID: WP_003102740.1
• Coordinates: 2022815..2023393 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HB729_RS09535
• Protein ID: WP_003088112.1
• Coordinates: 2023482..2024036 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB729_RS09515	2018091..2019368	+	1278	WP_003102743.1	TolC family protein	-
HB729_RS09520	2019358..2021529	+	2172	WP_009876279.1	type I secretion system permease/ATPase	-
HB729_RS09525	2021519..2022706	+	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HB729_RS09530	2022815..2023393	-	579	WP_003102740.1	HD domain-containing protein	Toxin
HB729_RS09535	2023482..2024036	-	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
HB729_RS09540	2024194..2026389	-	2196	WP_004349295.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HB729_RS09545	2026394..2026855	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HB729_RS09550	2027186..2027509	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
HB729_RS09555	2027522..2027920	+	399	WP_023082958.1	NADH-quinone oxidoreductase subunit A	-
HB729_RS09560	2027917..2028468	-	552	WP_003102727.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T152598 WP_003102740.1 NZ_CP050333:c2023393-2022815 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T152598 NZ_CP066343:c3019573-3019322 [Xanthomonas citri pv. durantae]
ATGCAACGGCTGCGCGAATTCCTGAAGCCGAAGAGTCCCGATGCCGCGAGGCGGGCCGGTGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGCCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGTGGCTATGTGGCTCGGTACCGCCACGCTGGCGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT152598 WP_003088112.1 NZ_CP050333:c2024036-2023482 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT152598 NZ_CP066343:c3019878-3019600 [Xanthomonas citri pv. durantae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCCGAGGCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGTGAGGCGTTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACGGGCCTGCACGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACAGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07