Detailed information of TA system TA152594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5669712..5670352
• Replicon: chromosome
• Accession: NZ_CP050332
• Organism: Pseudomonas aeruginosa strain DVT413

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HB730_RS26865
• Protein ID: WP_003105740.1
• Coordinates: 5669712..5670122 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: HB730_RS26870
• Protein ID: WP_003158175.1
• Coordinates: 5670122..5670352 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB730_RS26825	5665554..5665751	+	198	WP_003105756.1	hypothetical protein	-
HB730_RS26830	5665907..5666635	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
HB730_RS26835	5666641..5667189	+	549	WP_003105753.1	DUF3158 family protein	-
HB730_RS26840	5667236..5668075	+	840	WP_003105750.1	Rha family transcriptional regulator	-
HB730_RS26845	5668105..5668593	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
HB730_RS26850	5668701..5668946	+	246	WP_023102333.1	CrpP family protein	-
HB730_RS26855	5669012..5669230	-	219	WP_003105747.1	hypothetical protein	-
HB730_RS26860	5669430..5669690	-	261	WP_003105742.1	hypothetical protein	-
HB730_RS26865	5669712..5670122	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HB730_RS26870	5670122..5670352	-	231	WP_003158175.1	antitoxin	Antitoxin
HB730_RS26875	5670608..5672527	+	1920	WP_004352838.1	type I DNA topoisomerase	-
HB730_RS26880	5672835..5673044	+	210	WP_003105733.1	cold-shock protein	-
HB730_RS26885	5673265..5675154	+	1890	WP_003105732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5650141..5750531	100390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T152594 WP_003105740.1 NZ_CP050332:c5670122-5669712 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T152594 NZ_CP066337:c3409600-3408854 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCAGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT152594 WP_003158175.1 NZ_CP050332:c5670352-5670122 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT152594 NZ_CP066337:c3408853-3408683 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2