Detailed information of TA system TA152587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1337707..1338377
• Replicon: chromosome
• Accession: NZ_CP050332
• Organism: Pseudomonas aeruginosa strain DVT413

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A6M2XLD2
• Locus tag: HB730_RS06270
• Protein ID: WP_005934647.1
• Coordinates: 1337958..1338377 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HB730_RS06265
• Protein ID: WP_023114648.1
• Coordinates: 1337707..1337961 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB730_RS06245	1333647..1334648	-	1002	WP_058176106.1	hypothetical protein	-
HB730_RS06250	1334734..1334958	+	225	WP_009363662.1	AlpA family phage regulatory protein	-
HB730_RS06255	1334961..1335839	+	879	WP_058176105.1	helicase RepA family protein	-
HB730_RS06260	1335826..1336578	+	753	WP_058176104.1	hypothetical protein	-
HB730_RS06265	1337707..1337961	+	255	WP_023114648.1	Arc family DNA-binding protein	Antitoxin
HB730_RS06270	1337958..1338377	+	420	WP_005934647.1	type II toxin-antitoxin system VapC family toxin	Toxin
HB730_RS06275	1338534..1339304	+	771	WP_058176103.1	P-type conjugative transfer protein TrbJ	-
HB730_RS06280	1339315..1339473	+	159	WP_156797749.1	hypothetical protein	-
HB730_RS06285	1339475..1340944	+	1470	WP_058176102.1	P-type conjugative transfer protein TrbL	-
HB730_RS06290	1341013..1341255	+	243	WP_003137888.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
HB730_RS06295	1341267..1341548	+	282	WP_058176101.1	hypothetical protein	-
HB730_RS06300	1341551..1341919	+	369	WP_058176100.1	conjugal transfer transcriptional regulator TraJ	-
HB730_RS06305	1341957..1342415	-	459	WP_079760543.1	helix-turn-helix domain-containing protein	-
HB730_RS06310	1342412..1342588	-	177	WP_096222004.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1333647..1351176	17529

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14897.11 Da
• Isoelectric Point: 5.1890

>T152587 WP_005934647.1 NZ_CP050332:1337958-1338377 [Pseudomonas aeruginosa]
MIVLDTNVVSEAMKPEPNPAVRAWLNEQVAETLYLSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFGERVLMFDTE
AARHYAELAVKARTAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHQ

Nucleotide

• Length: 420 bp

>T152587 NZ_CP066312:c7562-7269 [Azotobacter chroococcum]
GTGCCACGAGTAGTCGTCACCGAAGGTGCAGCACAAGGTTTGGAACGCTGTCGCCTGTTCTTGGCTGAGAAAAGCCCGCA
GGTAGCTAGCCGTGCCGGCCAAGCCATCGAACATCAGTTCGCCCTCTTGGAGAAAAGCCCGGACATAGGGCGCCCATTTC
CTGAGCTGCCTGAGCTACGCGAGCTGATCATAGCCTTCGGTGACTCTGGCTATGTCGCGCTCTACCGATATGAGCCGGCG
GATGACACCGTGTACGTTTTGGCCTTCCGACATCAGAAAGAAGCAGGGTACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9508.87 Da
• Isoelectric Point: 6.4760

>AT152587 WP_023114648.1 NZ_CP050332:1337707-1337961 [Pseudomonas aeruginosa]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSEKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKVPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT152587 NZ_CP066312:c7822-7550 [Azotobacter chroococcum]
ATGGCGACTTCGATTAAAATCGACGATGAATTGAAAGCGCGTATCCAGCATCTGGCCTGCCAGCGGCAGCGCTCGGCCCA
CTGGATCATGCGCGAGGCAATCGCGCAGTATGTCGAGCGCGAGGAGGCTCGCGAGAGCTTCAAACAGGAGGCTTTGGCCT
CTTGGGCTGCCTACCGGGAAACCGGAAGGCATCTGACGGGCCAAGAAACGCGGGAATGGCTCAACACTTGGGGCAAGGAA
GAAGAAACGGCGGTGCCTGAGTGCCACGAGTAG

Structures

Toxin

• AlphaFold DB: A0A6M2XLD2