Detailed information of TA system TA152584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4619139..4619725
• Replicon: chromosome
• Accession: NZ_CP050331
• Organism: Pseudomonas aeruginosa strain DVT414

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HB731_RS21515
• Protein ID: WP_003120987.1
• Coordinates: 4619426..4619725 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HB731_RS21510
• Protein ID: WP_003448662.1
• Coordinates: 4619139..4619429 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB731_RS21490	4614290..4614499	+	210	WP_003105733.1	cold-shock protein	-
HB731_RS21495	4614721..4616610	+	1890	WP_016851610.1	hypothetical protein	-
HB731_RS21500	4616607..4618583	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
HB731_RS21505	4618724..4619068	+	345	WP_174816935.1	hypothetical protein	-
HB731_RS21510	4619139..4619429	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HB731_RS21515	4619426..4619725	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB731_RS21520	4619927..4621051	+	1125	WP_174816936.1	TcpQ domain-containing protein	-
HB731_RS21525	4621051..4622760	+	1710	WP_003123331.1	PilN family type IVB pilus formation outer membrane protein	-
HB731_RS21530	4622764..4624089	+	1326	WP_058152195.1	type 4b pilus protein PilO2	-
HB731_RS21535	4624079..4624612	+	534	WP_004352829.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4591318..4686549	95231

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T152584 WP_003120987.1 NZ_CP050331:c4619725-4619426 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T152584 NZ_CP066311:182628-183065 [Azotobacter chroococcum]
GTGATTCTGTGGCGGATCAGCGCCTACGCGGACCTCAGTGGAATCGGTGGCCTGCGTGCCAGTGGTCGCTGGCACCACCT
TGGTAGGCCGGTCGTTTACGCGGCGGACAGCCCGGCCGGTGCGATGCTGGAGGTGCTGGTCCATCTGGAAATCGACCCCG
AGGACTTCCCCACGACGCTCCAGTTGATCCGCGTCGAGCTTCCAGACCTGGTTTCGCGGGCCGAGCTTCCGCCGCTGCCG
GAGCACTGGAAATCCGACCCGGAGGTCACGCGCGCCATGGGTGATCGCTTTCTGGATGGGCGAAGCGCTCTGCTGCTCCC
GGTGCCGAGCGCCATCATTCCCTGCACGACCAACTACCTGTTCAACCCGGCGCACCCCGAGGCCAGTGGGGCGAGCATCC
AGGCAGAGAAATTCCCCCTCGATTCCCGCCTCATCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT152584 WP_003448662.1 NZ_CP050331:c4619429-4619139 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT152584 NZ_CP066311:182182-182631 [Azotobacter chroococcum]
ATGCTTGCTGAAGTCCTGCGTGACCATGGCTACACCGCATACCGGGCACGTCTTCAAAGCCTGCTGGACATCCCGGAGTC
GGCAACCGACTTTGAAATCCACACCCGGATCACCAAGGGCTTTCCTGCGACCCGCCTGGTGCAGCTGACCGAGGCCGGGG
TGGTCACGCCGCTGGAGCGCGATCAGATCATCCCCCTGAGAACCCTCAAGAGCCGCATCGAGCGCGATCAGCCGTTGACC
GTGGAGGAAAGCGACAGATTTTTTCGCGCTGCCCATATCACGGCAATGGCCGAGGCTGTCTTCGGCGACCGCGAGAAGGC
CAAACGTTGGCTCTCCAAGCCCAAGGAGCGGTTTTCCGGCCTGACGCCCATGCAGATGCTCACCACCCAGCAGGGCACCA
GCCAGGTGGAGGAAATGCTGCTGCAGATCGTCGAAGGTCTGGCCCTGTGA

Structures

Toxin

• AlphaFold DB: G8CP73