Detailed information of TA system TA152583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4611168..4611808
• Replicon: chromosome
• Accession: NZ_CP050331
• Organism: Pseudomonas aeruginosa strain DVT414

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HB731_RS21475
• Protein ID: WP_033993319.1
• Coordinates: 4611168..4611578 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6N0KGL5
• Locus tag: HB731_RS21480
• Protein ID: WP_023132185.1
• Coordinates: 4611578..4611808 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB731_RS21435	4607294..4607491	+	198	WP_003105756.1	hypothetical protein	-
HB731_RS21440	4607648..4608376	+	729	WP_073714124.1	TIGR03761 family integrating conjugative element protein	-
HB731_RS21445	4608382..4608930	+	549	WP_004352841.1	DUF3158 family protein	-
HB731_RS21450	4608977..4609816	+	840	WP_003105750.1	Rha family transcriptional regulator	-
HB731_RS21455	4609846..4610334	+	489	WP_004352840.1	single-stranded DNA-binding protein	-
HB731_RS21460	4610490..4610657	+	168	WP_128729268.1	CrpP family protein	-
HB731_RS21465	4610723..4610941	-	219	WP_033993321.1	hypothetical protein	-
HB731_RS21470	4610955..4611152	-	198	WP_003123325.1	hypothetical protein	-
HB731_RS21475	4611168..4611578	-	411	WP_033993319.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HB731_RS21480	4611578..4611808	-	231	WP_023132185.1	antitoxin	Antitoxin
HB731_RS21485	4612064..4613983	+	1920	WP_058149575.1	type I DNA topoisomerase	-
HB731_RS21490	4614290..4614499	+	210	WP_003105733.1	cold-shock protein	-
HB731_RS21495	4614721..4616610	+	1890	WP_016851610.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4591318..4686549	95231

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15422.76 Da
• Isoelectric Point: 7.3233

>T152583 WP_033993319.1 NZ_CP050331:c4611578-4611168 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVAPIV

Nucleotide

• Length: 411 bp

>T152583 NZ_CP066311:139876-140058 [Azotobacter chroococcum]
ATGCGAAGTCGGGAAGTGATCGAGCTGCTGGAGGCGGATGGTTGGTACGAAGTGGCGGTGAAAGGTAGTCACCACCAGTT
CAAGCACCCGACCAAGCCGGGGAGGGTGACGGTTCCGCACCCGAAATCGGATCTGCCGAAAGGCACCTGGCAGAGCATCC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8436.29 Da
• Isoelectric Point: 4.2821

>AT152583 WP_023132185.1 NZ_CP050331:c4611808-4611578 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTSDFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT152583 NZ_CP066311:140090-140494 [Azotobacter chroococcum]
ATGAAATATCCCGTCGTCATCCACAAAGAGCAGAGCAGCGACTACGGCGTGACCGTACCGGACGTGCCAGGCTGCTTCTC
GGCCGGCGAGACGATGACCGAAGCGCTGGAGAATGTCCGTGAGGCGCTGGAGTTGCATTTCGAGGGGCTGCTGGCCGATG
GCGAACCGCTGCCGCAGCCGCAGGAGATCGATGTGCACGCGGCGAACCCGGACTATGCCGGCGGCGTGTGGGCGCTGGTT
GACTTCGATGTGATGCCCTATCTGGGGAAGGCAGTGCGCTTCAACGCCACGCTGCCGGAGCACCTGCTGGCGCGGATCGA
TGCCTACGTGAAGGCCCATCCGGAGCAGAAGAGCCGCTCTGGATTTCTGGCTGAGGCGGCGCTGCGGGTGTTGCAGCACG
CTTGA

Structures

Antitoxin

• AlphaFold DB: A0A6N0KGL5