Detailed information of TA system TA152582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4543317..4544575
• Replicon: chromosome
• Accession: NZ_CP050331
• Organism: Pseudomonas aeruginosa strain DVT414

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HB731_RS21095
• Protein ID: WP_012614407.1
• Coordinates: 4543317..4544072 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HB731_RS21100
• Protein ID: WP_003086329.1
• Coordinates: 4544207..4544575 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB731_RS21085	4538490..4541033	+	2544	WP_033988132.1	acyl-homoserine-lactone acylase QuiP	-
HB731_RS21090	4541188..4542549	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
HB731_RS21095	4543317..4544072	-	756	WP_012614407.1	RES family NAD+ phosphorylase	Toxin
HB731_RS21100	4544207..4544575	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HB731_RS21105	4544648..4545934	-	1287	WP_012614408.1	FAD-binding oxidoreductase	-
HB731_RS21110	4545990..4547579	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
HB731_RS21115	4547657..4548133	+	477	WP_009877993.1	elongation factor GreAB	-
HB731_RS21120	4548136..4548411	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T152582 WP_012614407.1 NZ_CP050331:c4544072-4543317 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T152582 NZ_CP066311:111782-112123 [Azotobacter chroococcum]
ATGAGCTTCGACGTTCAGTTTGCTACTACTGCCGAAACCAGCCTGTTTTCCCAGATCGCCCACCTAGAGCCGTACCACAG
CTATGACGGGGCCTATGAAAAGCTCTCCACCTTGGTGGTAGAAGTTATACGCCGCTTGGCCGATCACCCGTTGGGCTACC
CCATCAGTGCACAAGTCAGCGAGCTTGGGATCGCCCATTACCGGGAGCTCAATCTCAACGGCTACCGCGTCTTCTATGAG
TGCGATGAAGAAAAGCGTCTCGTGGTGGTCCTGCTTATCCTGGGGCAGCGACAGAGCATCGAGCAGCAGCTGATCCACTA
CTGCCTGATGTTTGATCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT152582 WP_003086329.1 NZ_CP050331:c4544575-4544207 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT152582 NZ_CP066311:111525-111785 [Azotobacter chroococcum]
ATGAAAATCGAGACGATCAGCTTCTTGAAGAAGCACGCCGCATCGTTGGATCTGAGCGAGCCCATGGTGATCACCCAGAA
TGGTCTGCCCGCCTATGTCATCGAGTCCTATGCCGAACGCAAGCAGCGTGATGAGACGATTGCGCTCGTCAAGCTCCTGG
CATTGGGTTCCCGCGACAAAGAGCAAGGCAAGCTGGTGTCGGGTGACACGCTCCTGGATCGGTTGGCACAACGATGCCAG
CAACCCAAAGGAACTTCATGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7