Detailed information of TA system TA152576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4517291..4518549
• Replicon: chromosome
• Accession: NZ_CP050330
• Organism: Pseudomonas aeruginosa strain DVT779

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HB733_RS20960
• Protein ID: WP_023084953.1
• Coordinates: 4517291..4518046 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HB733_RS20965
• Protein ID: WP_003086329.1
• Coordinates: 4518181..4518549 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB733_RS20950	4512464..4515007	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
HB733_RS20955	4515162..4516523	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
HB733_RS20960	4517291..4518046	-	756	WP_023084953.1	RES family NAD+ phosphorylase	Toxin
HB733_RS20965	4518181..4518549	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HB733_RS20970	4518622..4519908	-	1287	WP_057393369.1	FAD-binding oxidoreductase	-
HB733_RS20975	4519964..4521553	-	1590	WP_079743354.1	aldehyde dehydrogenase family protein	-
HB733_RS20980	4521631..4522107	+	477	WP_003086322.1	hypothetical protein	-
HB733_RS20985	4522110..4522385	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28310.49 Da
• Isoelectric Point: 7.7294

>T152576 WP_023084953.1 NZ_CP050330:c4518046-4517291 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGMRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T152576 NZ_CP066310:c1743387-1743196 [Azotobacter chroococcum]
ATGAAATACAGCGAGTTCCGGCGATGGCTGGAGGCCCAAGGGGTTGAGTTCACGAAGTCGCGCGGCGGTAGCAGCCACTT
CAAGATCAAAGCCCCGAACGGCAAACAGACGATCTTCCCGTATCACGGAGCCAAGGAAATCGGCGAAGGACTCAGGAAGG
ACATCATCAAGCAGCTGGGCCTCAACGACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT152576 WP_003086329.1 NZ_CP050330:c4518549-4518181 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT152576 NZ_CP066310:c1743144-1742722 [Azotobacter chroococcum]
ATGTACGACTATCCGATCACCGTGCACAGCGAAGCCGGCCATTATTGGTCGTCTTGCCCGGACATTCCGGAAGCTCACAG
CGCAGGCGATACCATTGGGATGCTGCTGGCCAATGCCTTCGATGGAATCCGCCTCGCGCTGACCATCTACGTTGACCAGG
GAAGAAGCATTCCACCCGCTTCTCCTGCCAAGGCAGGCCAATACGTTGTTTCACTCCCGGCGCAGACGGTGGCCAAAGTG
CACCTATGGAACACGATGCGCGAACAGGGGCTGCGGGTTGCAGACTTGGCCAGATTGCTTGGCGTCTCTCATCCCGTGGC
TACCCGACTGGTCGACTTCGAGCACGTCTCGAAGATCGAGCAGATTGAGCGAGCCTTGCATGCTCTCGGAAAGCGTCTGT
CTGTTTCCGTCGAGGCAGCATGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7