Detailed information of TA system TA152574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1875824..1877332
• Replicon: chromosome
• Accession: NZ_CP050330
• Organism: Pseudomonas aeruginosa strain DVT779

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HB733_RS08815
• Protein ID: WP_073666805.1
• Coordinates: 1875824..1876411 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX2
• Locus tag: HB733_RS08820
• Protein ID: WP_003115566.1
• Coordinates: 1876478..1877332 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB733_RS08810	1872079..1875558	-	3480	WP_003117605.1	hybrid sensor histidine kinase/response regulator	-
HB733_RS08815	1875824..1876411	+	588	WP_073666805.1	GNAT family N-acetyltransferase	Toxin
HB733_RS08820	1876478..1877332	+	855	WP_003115566.1	AraC family transcriptional regulator	Antitoxin
HB733_RS08825	1877429..1879594	+	2166	WP_025271210.1	TonB-dependent receptor family protein	-
HB733_RS08830	1879769..1881643	+	1875	WP_003117607.1	MFS transporter	-
HB733_RS08835	1881704..1881913	-	210	WP_003115568.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21887.20 Da
• Isoelectric Point: 9.8676

>T152574 WP_073666805.1 NZ_CP050330:1875824-1876411 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLADQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T152574 NZ_CP066310:1515887-1516141 [Azotobacter chroococcum]
TTGAACATTCTCTTCACGCCCGAGGCCTGGGACGACTACCTGTGGTTCCAGCAAAACGACAAGGCCGGGCTCAAGCGGAT
CAACCTGCTGATCCGGGATGCGCAACGCGATCCGTTCGGCGGTCTTGGCAAGCCGGAGCCCTTGAAGCACAACCTCAGCG
GTTTCTGGTCGCGCCGCATCGGCGAGGAACATCGGCTGGTCTACAGGGTGGACGGAGATGCTCTGCAGATCGTGATGTGC
CGTTATCACTACTGA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31933.23 Da
• Isoelectric Point: 7.3276

>AT152574 WP_003115566.1 NZ_CP050330:1876478-1877332 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 855 bp

>AT152574 NZ_CP066310:1515626-1515883 [Azotobacter chroococcum]
ATGCAGACCATCAACTACACCACGGCACGCCAGCACCTGGCGGAAACCATGGATCGGGTGAACAACGACCGCACGCCCAT
CCTGGTCACCCGGCAGAAGGGCGAGCCGGTGGTGATGATTTCCCTAGCCGACTACAACGCTCTCGAGGAGACGGCCTATC
TGCTGCGCTCGCCGGCCAATGCGGAGCGTCTGATCCGCTCGATCGCCAGCCTGCGCGCCGGCAAGGCGGAGCCGCGGTCG
CTCGTCGACGAAGGCTGA

Structures

Antitoxin

• AlphaFold DB: Q9HYX2