Detailed information of TA system TA152569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3524690..3525911
• Replicon: chromosome
• Accession: NZ_CP050329
• Organism: Pseudomonas aeruginosa strain DVT417

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HB734_RS16225
• Protein ID: WP_003114949.1
• Coordinates: 3525333..3525911 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HB734_RS16220
• Protein ID: WP_003088112.1
• Coordinates: 3524690..3525244 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB734_RS16195	3520258..3520809	+	552	WP_003102727.1	XRE family transcriptional regulator	-
HB734_RS16200	3520806..3521204	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HB734_RS16205	3521217..3521540	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
HB734_RS16210	3521871..3522332	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HB734_RS16215	3522337..3524532	+	2196	WP_003122807.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HB734_RS16220	3524690..3525244	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
HB734_RS16225	3525333..3525911	+	579	WP_003114949.1	HD domain-containing protein	Toxin
HB734_RS16230	3526019..3527206	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HB734_RS16235	3527196..3529367	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
HB734_RS16240	3529357..3530634	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21897.75 Da
• Isoelectric Point: 5.3655

>T152569 WP_003114949.1 NZ_CP050329:3525333-3525911 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAVRRDA

Nucleotide

• Length: 579 bp

>T152569 NZ_CP066307:42552-42989 [Pseudomonas putida]
ATGAGCAAAAAGACGTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGCCCCGACGCGGTGCTGGCCAA
GCTGGAACAGGCAGTCACTAATCAGCACCGGATTGTCGTTTCGGCCATCACCTACTCCGAAATGCGTTTCGGGGCCGCCA
ACCCCAAGGCATCGCCCAAGGTTGCAGCGATGGTCGATGCCTTCATCCAGCGCCTGGACGCCATCCTGTCCTGGGACGCC
GCCGCCGTAGATCAAACAACGCAGATCCGCACCGCCCTCGCCCGCCTGGGTACGCCCATTGGCAACAACGATGCAGCGAT
AGCCGGCCATGCCCTGGCCGCTGGGTGTGTTCTGGTCACGAACAACACTCGCGAATTTGCTCGCGTTCCTGGCCTAGTCC
TTGAGGACTGGACCCAACCCGCCATTACCAAGAGTTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT152569 WP_003088112.1 NZ_CP050329:3524690-3525244 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT152569 NZ_CP066307:42319-42555 [Pseudomonas putida]
ATGCGTACCGTTTCCATTTTCATGAACAGCCGAAACCAGGCGATCCGCATCCCTCGGGATATGGAGTACCAGGGCGTTTC
CGAGCTGGAGATTATCCGCGACGGCGAAACGCTGATCCTGCGCCCTGTGCGCCCCTCCTGGGCATCGTTGCGCGACGTGG
CGGCCGCAGATCCTGACTTTCTGACCGAGCGAAAGGATGTGATCGAGGAAGACCGTTTCCTGGGTGACTTCACATGA