Detailed information of TA system TA152565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 3903795..3905303
• Replicon: chromosome
• Accession: NZ_CP050328
• Organism: Pseudomonas aeruginosa strain DVT419

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HB736_RS18405
• Protein ID: WP_079276297.1
• Coordinates: 3904716..3905303 (-)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A5B3ABU1
• Locus tag: HB736_RS18400
• Protein ID: WP_003119683.1
• Coordinates: 3903795..3904649 (-)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB736_RS18385	3899214..3899423	+	210	WP_003115568.1	cold-shock protein	-
HB736_RS18390	3899484..3901358	-	1875	WP_003117607.1	MFS transporter	-
HB736_RS18395	3901533..3903698	-	2166	WP_003115567.1	TonB-dependent receptor family protein	-
HB736_RS18400	3903795..3904649	-	855	WP_003119683.1	AraC family transcriptional regulator	Antitoxin
HB736_RS18405	3904716..3905303	-	588	WP_079276297.1	GNAT family N-acetyltransferase	Toxin
HB736_RS18410	3905569..3909048	+	3480	WP_003117605.1	hybrid sensor histidine kinase/response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21892.22 Da
• Isoelectric Point: 9.8675

>T152565 WP_079276297.1 NZ_CP050328:c3905303-3904716 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQQGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T152565 NZ_CP066303:c1655887-1655678 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTATAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31875.19 Da
• Isoelectric Point: 7.5295

>AT152565 WP_003119683.1 NZ_CP050328:c3904649-3903795 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 855 bp

>AT152565 NZ_CP066303:c1656187-1655894 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A5B3ABU1