Detailed information of TA system TA152562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 981168..981808
• Replicon: chromosome
• Accession: NZ_CP050328
• Organism: Pseudomonas aeruginosa strain DVT419

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HB736_RS04630
• Protein ID: WP_003134109.1
• Coordinates: 981398..981808 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HB736_RS04625
• Protein ID: WP_031634724.1
• Coordinates: 981168..981398 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB736_RS04615	978213..978965	-	753	WP_023103392.1	hypothetical protein	-
HB736_RS04620	978973..980892	-	1920	WP_023103391.1	type I DNA topoisomerase	-
HB736_RS04625	981168..981398	+	231	WP_031634724.1	antitoxin	Antitoxin
HB736_RS04630	981398..981808	+	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HB736_RS04635	981824..982018	+	195	WP_023103390.1	hypothetical protein	-
HB736_RS04640	982320..982496	-	177	WP_126546597.1	CrpP family protein	-
HB736_RS04645	982708..983196	-	489	WP_023103388.1	single-stranded DNA-binding protein	-
HB736_RS04650	983210..983743	-	534	WP_023103387.1	DUF3158 family protein	-
HB736_RS04655	983749..984477	-	729	WP_023103386.1	TIGR03761 family integrating conjugative element protein	-
HB736_RS04660	984470..984889	-	420	WP_140419861.1	hypothetical protein	-
HB736_RS04665	984918..985157	-	240	WP_003149503.1	hypothetical protein	-
HB736_RS04670	985148..985393	-	246	WP_023103385.1	hypothetical protein	-
HB736_RS04675	985390..986715	-	1326	WP_023103384.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	887124..999929	112805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T152562 WP_003134109.1 NZ_CP050328:981398-981808 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T152562 NZ_CP066294:1720655-1720987 [Streptococcus mutans]
ATGGTAACCATCAAGCAAGGGTCAATTATCAAAATTAACTTGGTTCCCAAACAAGGACATGAACAAAAAGGGTATCGTCC
GTACATTTGTCTAAACCATAGTATCGTAACCAAGTATTCTAATATTGCTATTTTTGCGCCAATTAGCAATACCAAGCGTG
ATTACCCTTTTTATGTTCCCCTAGAAGGAACAGAATCCACAGGGAAAGTATTATTAGACCAACTGGTTACAATCGATTTT
AATGCTAGAGATTATCGTTATGTGGAGGATATTCAGGAAGACTTATTAGATGAACTTTTAGCTAGGGTCAAGGTGCTATT
TGAAAAAGGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT152562 WP_031634724.1 NZ_CP050328:981168-981398 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT152562 NZ_CP066294:1720416-1720661 [Streptococcus mutans]
ATGCAACTAGTCATCAATAAATGGGGAAATAGCTCCGCTATTCGTTTGCCAAAACAATTGGTACAAGAATTACAATTACA
AACAAATGATGTCTTAGACTATAAGGTATCAGGGAATAAAATTATCCTAGAAAAAGTGAATAATGTCCCTGAATTAACTG
TAGAAGATCTGTTTAAAGATTATCAAGGTGAACCTGTCAATGTTACCCCAGTTCTATTTGAAAGTGTGGGAAATGAACAA
TGGTAA