Detailed information of TA system TA152553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5217229..5217815
• Replicon: chromosome
• Accession: NZ_CP050325
• Organism: Pseudomonas aeruginosa strain DVT425

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HB740_RS24255
• Protein ID: WP_003120987.1
• Coordinates: 5217516..5217815 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HB740_RS24250
• Protein ID: WP_003448662.1
• Coordinates: 5217229..5217519 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB740_RS24230	5212380..5212589	+	210	WP_003105733.1	cold-shock protein	-
HB740_RS24235	5212811..5214700	+	1890	WP_016851610.1	hypothetical protein	-
HB740_RS24240	5214697..5216673	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
HB740_RS24245	5216814..5217158	+	345	WP_016851612.1	hypothetical protein	-
HB740_RS24250	5217229..5217519	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HB740_RS24255	5217516..5217815	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB740_RS24260	5218017..5219141	+	1125	WP_034024019.1	TcpQ domain-containing protein	-
HB740_RS24265	5219141..5220850	+	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
HB740_RS24270	5220854..5222179	+	1326	WP_031806139.1	type 4b pilus protein PilO2	-
HB740_RS24275	5222169..5222702	+	534	WP_023094340.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5189834..5323960	134126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T152553 WP_003120987.1 NZ_CP050325:c5217815-5217516 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T152553 NZ_CP066268:c72061-71684 [Pseudomonas tremae]
GTGAAAGGCGTCCTGGTAGACACCTCTGTTTGGGTTGAGCACTTTCGCAACAACAGTCCAGAACTGGTGAACTTGCTCAG
CCAGGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCGCCTGATCGTTCGAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATCGCCTTTCTGAACACCCACAAGCTCTAC
GGCCTGGGCTGTGGGCTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGCCGCATGGCCGTGTCATACCAGCCTCCTACTCACTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT152553 WP_003448662.1 NZ_CP050325:c5217519-5217229 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT152553 NZ_CP066268:c72258-72058 [Pseudomonas tremae]
ATGAGAACGACCGTCACCATCGACGATGCGTTGTACCAACGTGCTCTGGAAGTCGCCGATCCGGCGATGGACAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCAGCTAAACGATTGATGGCCTTAGGGGCCACACTGCCAA
CGATGGAAGACATCGCCCGCCGACACGAGAAGGCACTGTGA

Structures

Toxin

• AlphaFold DB: G8CP73