Detailed information of TA system TA152552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5209258..5209898
• Replicon: chromosome
• Accession: NZ_CP050325
• Organism: Pseudomonas aeruginosa strain DVT425

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HB740_RS24215
• Protein ID: WP_033993319.1
• Coordinates: 5209258..5209668 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6N0KGL5
• Locus tag: HB740_RS24220
• Protein ID: WP_023132185.1
• Coordinates: 5209668..5209898 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB740_RS24175	5205019..5205216	+	198	WP_003105756.1	hypothetical protein	-
HB740_RS24180	5205372..5206100	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
HB740_RS24185	5206106..5206654	+	549	WP_033973539.1	DUF3158 family protein	-
HB740_RS24190	5206702..5207532	+	831	WP_025297978.1	Rha family transcriptional regulator	-
HB740_RS24195	5207562..5208050	+	489	WP_058149574.1	single-stranded DNA-binding protein	-
HB740_RS24200	5208580..5208747	+	168	WP_147318654.1	CrpP family protein	-
HB740_RS24205	5208813..5209031	-	219	WP_033993321.1	hypothetical protein	-
HB740_RS24210	5209045..5209242	-	198	WP_003123325.1	hypothetical protein	-
HB740_RS24215	5209258..5209668	-	411	WP_033993319.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HB740_RS24220	5209668..5209898	-	231	WP_023132185.1	antitoxin	Antitoxin
HB740_RS24225	5210154..5212073	+	1920	WP_073660273.1	type I DNA topoisomerase	-
HB740_RS24230	5212380..5212589	+	210	WP_003105733.1	cold-shock protein	-
HB740_RS24235	5212811..5214700	+	1890	WP_016851610.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5189834..5323960	134126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15422.76 Da
• Isoelectric Point: 7.3233

>T152552 WP_033993319.1 NZ_CP050325:c5209668-5209258 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVAPIV

Nucleotide

• Length: 411 bp

>T152552 NZ_CP066267:5869603-5869878 [Pseudomonas tremae]
ATGGAATTGAAGTGGACAAGCAAGGCGCTTTCCGACATTACCCGGCTATACGAGTTTCTGGCCTCTGTGAATCAACCTGC
TGCCGCACGAGCAGTGCAGCAACTCACCACCGCACCTACCACGCTGCTGACCAATCCGCGCATTGGAGAACGCCTGGAGG
AATTCGAGCCAAGGGACGTACGCCGAATTCAGATCGGCCAATACGAGATGCGTTACGAGATAGCGGATTCCACTATCTAC
CTGCTGCGCCTGTGGCACACCCGCGAAGACCGATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8436.29 Da
• Isoelectric Point: 4.2821

>AT152552 WP_023132185.1 NZ_CP050325:c5209898-5209668 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTSDFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT152552 NZ_CP066267:5869331-5869603 [Pseudomonas tremae]
ATGGCAACCACCGCCAAAACCCGATCCGTCACAGCTCACGTTCCGGTGCAACTGGCGGAAAAAGTCGATTTGATGGCTGA
GCGTCTGGAGCGCTCCAAGAACTGGATCGTCAAGCAAGCTCTGTCCGCTTGGATAGACCAAGAAGAAGAGCGTAGTCGCC
TGACCCGTGAGGCTTTGGCTGATGTTGACGCTGGACGCGTTATTGACCACCAAGCCGTCCAGGCCTGGGCTGACAGTCTC
AGCACCGACACTCCGTTGCCGGTGCCGCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6N0KGL5