Detailed information of TA system TA152549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4508177..4509435
• Replicon: chromosome
• Accession: NZ_CP050325
• Organism: Pseudomonas aeruginosa strain DVT425

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HB740_RS20840
• Protein ID: WP_023084953.1
• Coordinates: 4508177..4508932 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HB740_RS20845
• Protein ID: WP_003086329.1
• Coordinates: 4509067..4509435 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB740_RS20830	4503350..4505893	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
HB740_RS20835	4506048..4507409	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
HB740_RS20840	4508177..4508932	-	756	WP_023084953.1	RES family NAD+ phosphorylase	Toxin
HB740_RS20845	4509067..4509435	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HB740_RS20850	4509508..4510794	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
HB740_RS20855	4510850..4512439	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
HB740_RS20860	4512518..4512994	+	477	WP_003140638.1	hypothetical protein	-
HB740_RS20865	4512997..4513272	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28310.49 Da
• Isoelectric Point: 7.7294

>T152549 WP_023084953.1 NZ_CP050325:c4508932-4508177 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGMRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T152549 NZ_CP066265:1343151-1343333 [Pseudomonas syringae]
GTGCAGAGCAGGCATTTGATGAAGGAGCTTGAAGCACACGGGTGGATTCTTGATCGCGTTACCGGCAGTCATCATATGTT
CAAACATCCGCAAAAAGCTCAGACAGTTCCAGTGCCACATCCCAAGAAGGATTTGCCAGTGGGTACGGTGAAAGCCATCA
GAAAGCTGGCCGGATTGGTGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT152549 WP_003086329.1 NZ_CP050325:c4509435-4509067 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT152549 NZ_CP066265:1343369-1343773 [Pseudomonas syringae]
ATGAAATATCCAATGTGCATAGAGTGGGGAGATGAAACCACAGCGTTCGGTATCCAGATCCCTGACATTCCCGGCGCAAT
CAGTGCAGGCGATACCTTCGAGCAAGCTCATGCGGCGGCCGTGGAGATTGCGCATATCATGCTGCAAGAGATCGCCGCCA
GTGGTGGAACCCTCCCCAAGGCCAGGACCGTTGCCGAGCATGCCAAAAACCCTGACTTCTCCGGAATGGGCTGGGGCATG
CTCGAAATCGATATAACCCTTTATCTGGGAAAGACCGAGAAAGTCAACGTCACCTTGCCGGGTTTCGTCATCAAGCAGAT
CGACCGCTATGTGCGGGATCACAGCATCAAGAGTCGCTCGACCTTCCTCGCCGATGCCGCGCTTGAAAAACTTGGTCGGG
CGTAA

Structures

Antitoxin

• AlphaFold DB: V6AJU7