Detailed information of TA system TA152543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4560949..4561589
• Replicon: chromosome
• Accession: NZ_CP050324
• Organism: Pseudomonas aeruginosa strain DVT427

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HB742_RS21065
• Protein ID: WP_003105740.1
• Coordinates: 4560949..4561359 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: HB742_RS21070
• Protein ID: WP_003158175.1
• Coordinates: 4561359..4561589 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB742_RS21025	4556791..4556988	+	198	WP_003105756.1	hypothetical protein	-
HB742_RS21030	4557144..4557872	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
HB742_RS21035	4557878..4558426	+	549	WP_003105753.1	DUF3158 family protein	-
HB742_RS21040	4558473..4559312	+	840	WP_003105750.1	Rha family transcriptional regulator	-
HB742_RS21045	4559342..4559830	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
HB742_RS21050	4559938..4560183	+	246	WP_023102333.1	CrpP family protein	-
HB742_RS21055	4560249..4560467	-	219	WP_003105747.1	hypothetical protein	-
HB742_RS21060	4560667..4560927	-	261	WP_003105742.1	hypothetical protein	-
HB742_RS21065	4560949..4561359	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HB742_RS21070	4561359..4561589	-	231	WP_003158175.1	antitoxin	Antitoxin
HB742_RS21075	4561845..4563764	+	1920	WP_004352838.1	type I DNA topoisomerase	-
HB742_RS21080	4564072..4564281	+	210	WP_003105733.1	cold-shock protein	-
HB742_RS21085	4564502..4566391	+	1890	WP_003109354.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4541378..4641366	99988

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T152543 WP_003105740.1 NZ_CP050324:c4561359-4560949 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T152543 NZ_CP066256:c53237-53079 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT152543 WP_003158175.1 NZ_CP050324:c4561589-4561359 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT152543 NZ_CP066256:c53505-53281 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin

• AlphaFold DB: Q6X2S2